Fck1, a C-type cyclin-dependent kinase, interacts with Fcc1 to regulate development and secondary metabolism in Fusarium verticillioides
Fck1, a C-type cyclin-dependent kinase, interacts with Fcc1 to regulate development and secondary metabolism in Fusarium verticillioides
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DOI：
10.1016/j.fgb.2005.09.006
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发表时间：
2006-03-01
影响因子：
3
通讯作者：
Woloshuk, CP
中科院分区：
文献类型：
--
作者：
Bluhm, BH;Woloshuk, CP
在轮枝镰刀菌中，C型细胞周期蛋白Fcc 1是基因表达的全局调节因子。在酿酒酵母和其他生物体中，C型细胞周期蛋白通过物理缔合调节特定细胞周期蛋白依赖性激酶的活性。我们确定了FCK 1，一个基因编码的细胞周期蛋白依赖性激酶在轮枝镰刀菌。预测Fck 1蛋白含有与先前描述的细胞周期蛋白依赖性激酶同源的细胞周期蛋白结合基序和丝氨酸-苏氨酸蛋白激酶结构域。FCK 1的破坏导致多效性形态缺陷，包括生长减少、分生孢子形成、伏马菌素B-1（FB 1）产生和色素沉着增强。双杂交分析表明Fcc 1和Fck 1之间存在较强的物理相互作用。本研究首次描述了丝状真菌中C型细胞周期蛋白和细胞周期蛋白依赖性激酶之间的相互作用，并提供了关于调控F.轮枝状(c)2006爱思唯尔公司All rights reserved.
In Fusarium verticillioides, the C-type cyclin Fcc1 is a global regulator of gene expression. In Saccharomyces cerevisiae and other organisms, C-type cyclins regulate the activity of specific cyclin-dependent kinases through physical association. We identified FCK1, a gene encoding a cyclin-dependent kinase in F verticillioides. The Fck1 protein is predicted to contain a cyclin-binding motif and a serine-threonine protein kinase domain homologous to previously described cyclin-dependent kinases. Disruption of FCK1 resulted in pleiotropic morphological defects including reduced growth, conidiation, fumonisin B-1 (FB1) production, and enhanced pigmentation. Two-hybrid analysis indicated a strong physical interaction between Fcc1 and Fck1. This study presents the first description of the interaction between a C-type cyclin and a cyclin-dependent kinase in a filamentous fungus and provides new insight regarding a molecular mechanism that regulates aspects of maize kernel colonization by F. verticillioides. (c) 2006 Elsevier Inc. All rights reserved.