Rapid and specific sensing of tetracycline in food using a novel upconversion aptasensor
Rapid and specific sensing of tetracycline in food using a novel upconversion aptasensor
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使用新型上转换适体传感器快速、特异性地检测食品中的四环素
DOI：
10.1016/j.foodcont.2017.06.004
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发表时间：
2017-11-01
期刊：
影响因子：
6
通讯作者：
Hu, Weiwei
中科院分区：
文献类型：
--
作者：
Ouyang, Qin;Liu, Yan;Hu, Weiwei
稀土离子掺杂的上转换纳米粒子（UCNPs）由于其独特的频率上转换能力和高的检测灵敏度，在生物检测领域具有广阔的应用前景。本研究开发了一种新型、灵敏的基于适配体的UCNPs（NaY（0.48）Gd（0.3）F4：Yb-0.2，Homy）生物传感器，用于检测食品中的四环素（泰特）。制备了适体-磁性纳米颗粒（aptamer-MNP）和互补DNA-UCNPs（cDNA-UCNPs）缀合物，并分别用作捕获和信号探针。它们形成了MNP-适体-cDNA-UCNPs的复合物，并完全结合。加入泰特后，适体优先与泰特结合，导致部分cDNA解离，部分cDNA-UCNPs释放。这导致MNP表面上的荧光信号降低。在优化条件下，泰特的检测线性范围为0.01 ~ 100 ng/mL，检测限为0.0062 ng/mL。该方法已成功应用于污染样品中泰特的测定。研究结果表明，该生物传感器为食品中泰特的检测提供了一种高效、特异、简便的方法，在食品安全和质量控制方面具有很大的应用潜力。(C)2017爱思唯尔有限公司版权所有
Rare earth-doped upconversion nanoparticles (UCNPs) have a promising potential in biodetection due to their unique frequency upconverting capability and high detection sensitivity. This study developed a novel and sensitive aptamer-based UCNPs (NaY(0.48)Gd(0.3)F4:Yb-0.2, Homy) biosensor for sensing tetracycline (TET) in foodstuffs. The aptamer-magnetic nanoparticles (aptamer-MNPs) and the complementary DNA-UCNPs (cDNA-UCNPs) conjugates were prepared and used as the capture and signal probes, respectively. They formed the complex of MNPs-aptamer-cDNA-UCNPs with complete binding. With TET addition, the aptamer preferentially bound with TET and caused the dissociation of some cDNA; and the liberation of some cDNA-UCNPs. This led to a decreased fluorescent signal on the surface of MNPs. Under the optimal conditions, a wide linear detection range from 0.01 to 100 ng/mL was achieved, with a limit of detection of 0.0062 ng/mL, for detection of TET. The proposed method was successfully applied to measure TET in contaminated samples. Results showed that the proposed UCNPs biosensor offers an efficient, specific, and simple approach for the detection of TET in food, and has high potential for food safety and quality control. (C) 2017 Elsevier Ltd. All rights reserved.