Expression of members of the 28-kilodalton major outer membrane protein family of Ehrlichia chaffeensis during persistent infection.
Expression of members of the 28-kilodalton major outer membrane protein family of Ehrlichia chaffeensis during persistent infection.
复制标题
查菲埃里希体 28 千道尔顿主要外膜蛋白家族成员在持续感染过程中的表达。
DOI：
10.1128/iai.72.8.4336-4343.2004
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Yu,Xue-jie
中科院分区：
文献类型：
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作者：
Zhang,Jian-zhi;Guo,Hong;Winslow,GaryM;Yu,Xue-jie
chaffenensis衣著体的28 kda免疫显性外膜蛋白（P28 OMPs）是由一个多基因家族编码的。作为一种间接测量p28多基因家族成员在体内表达的方法。chaffeensis，来自两只实验感染的比格犬的血清。采用酶联免疫吸附法对沙芬虫进行P28 OMPs特异性抗体的检测。在每个P28 OMP的第一个高变区中鉴定出每个P28所特有的抗原肽。从接种后第30天到第468天，从第46天到第159天，检测了两只小猎犬对所有P28 OMPs衍生肽的血清学反应。虽然对肽的抗体滴度波动，但对所有肽的峰值反应同时出现在每只狗身上。抗体应答另一种外膜蛋白e。chaffeensis（GP120）表现出相似的时间和数量变化。这些数据表明P28 OMPs在持续感染期间同时表达。
The 28-kDa immunodominant outer membrane proteins (P28 OMPs) ofEhrlichia chaffeensisare encoded by a multigene family. As an indirect measure of the in vivo expression of the members of thep28multigene family ofE. chaffeensis, sera from two beagle dogs experimentally infected withE. chaffeensiswere evaluated for the presence of specific antibodies to P28 OMPs by enzyme-linked immunosorbent assay. Antigenic peptides unique to each of the P28s were identified within the first hypervariable region of each P28 OMP. Serological responses to peptides derived from all P28 OMPs were detected from day 30 postinoculation to day 468 and from day 46 until day 159 in the two beagles. Although antibody titers to the peptides fluctuated, the peak response to all of the peptides appeared simultaneously in each dog. The antibody responses to another outer membrane protein ofE. chaffeensis(GP120) showed similar temporal and quantitative changes. These data suggest that the P28 OMPs are expressed concurrently during persistentEhrlichiainfection.