Microarray studies of gene expression in fish
Microarray studies of gene expression in fish
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DOI：
10.1089/omi.2006.10.474
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发表时间：
2006-12-01
影响因子：
3.3
通讯作者：
Douglas, Susan E.
中科院分区：
文献类型：
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作者：
Douglas, Susan E.
近年来，微阵列技术在鱼类生理学各个方面的研究有了惊人的增长。从早期对模式物种的研究，如斑马鱼，到目前对商业上重要的物种的研究，如鲑鱼、鲶鱼、鲤鱼和比目鱼，微阵列技术已经成为理解发育过程和基本生理学的关键工具。此外，微阵列技术还被应用于生态毒理学和营养基因组学等领域。现在有许多不同的平台可供选择，从含有cDNA扩增子的微阵列到各种大小的低聚物。高密度微阵列包含成千上万种不同的寡聚物已经开发用于斑马鱼和鲶鱼。随着这项令人兴奋的技术的进步，我们对鱼类整体基因表达的理解也将随之发展。此外，从这种实验上易于处理的生物群体中吸取的经验教训也可以应用于更高级的生物，如人类。
The use of microarrays for the study of various aspects of fish physiology has seen a spectacular increase in recent years. From early studies with model species, such as zebrafish, to current studies with commercially important species, such as salmonids, catfish, carp, and flatfish, microarray technology has emerged as a key tool for understanding developmental processes as well as basic physiology. In addition, microarrays are being applied to the fields of ecotoxicology and nutrigenomics. A number of different platforms are now available, ranging from microarrays containing cDNA amplicons to oligomers of various sizes. High-density microarrays containing hundreds of thousands of distinct oligomers have been developed for zebrafish and catfish. As this exciting technology advances, so will our understanding of global gene expression in fish. Furthermore, lessons learned from this experimentally tractable group of organisms can also be applied to more advanced organisms such as humans.