Systemic oxidative stress in asthma, COPD, and smokers
Systemic oxidative stress in asthma, COPD, and smokers
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DOI：
10.1164/ajrccm.154.4.8887607
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发表时间：
1996-10-01
影响因子：
24.7
通讯作者：
MacNee, W
中科院分区：
文献类型：
--
作者：
Rahman, I;Morrison, D;MacNee, W
在吸烟者和呼吸道疾病患者中，氧化剂和抗氧化剂之间存在不平衡。我们通过测量血浆的Trolox当量抗氧化能力（TEAC）和作为整体氧化应激指标的脂质过氧化产物水平来验证这一假设。与健康的非吸烟者（1.31 +/- 0.10 mmol/L, n = 14, p < 0.001）相比，健康的慢性吸烟者血浆TEAC明显降低（0.66 +/- 0.07 mmol/L，平均+/- SEM， n = 11），脂质过氧化产物水平增加，在研究前1小时吸烟的人群中，这种影响被放大了。慢性阻塞性肺疾病（COPD）急性加重（0.46 +/- 0.10 mmol/L, n = 20, p < 0.001）或哮喘（0.61 +/- 0.05 mmol/L, n = 9, p < 0.01）患者血浆TEAC也较低，且血浆脂质过氧化产物升高。慢性阻塞性肺病急性加重患者受刺激的中性粒细胞释放超氧阴离子与血浆抗氧化能力呈负相关（r = -0.73, p < 0.001）。TEAC的显著降低与COPD急性加重期血浆蛋白巯基浓度降低有关，但与吸烟者或哮喘患者无关。因此，吸烟、慢性阻塞性肺病急性加重和哮喘与血液中明显的氧化剂/抗氧化剂失衡有关，与氧化应激增加的证据有关。在这些条件下，血浆抗氧化能力的下降可能有不同的机制。
An imbalance between oxidants and antioxidants is proposed in smokers and in patients with airways diseases. We tested this hypothesis by measuring the Trolox equivalent antioxidant capacity (TEAC) of plasma and the levels of products of lipid peroxidation as indices of overall oxidative stress. The plasma TEAC was markedly reduced (0.66 +/- 0.07 mmol/L; mean +/- SEM; n = 11), with increased levels of lipid peroxidation products, in healthy chronic smokers as compared with healthy nonsmokers (1.31 +/- 0.10 mmol/L, n = 14, p < 0.001), an effect that was exaggerated in those who had smoked 1 h before the study. Plasma TEAC was also low in patients presenting with acute exacerbations of chronic obstructive pulmonary disease (COPD) (0.46 +/- 0.10 mmol/L, n = 20, p < 0.001) or asthma (0.61 +/- 0.05 mmol/L, n = 9, p < 0.01) with increases in plasma lipid peroxidation products. There was a negative correlation between superoxide anion release by stimulated neutrophils and plasma antioxidant capacity (r = -0.73, p < 0.001) in patients with acute exacerbations of COPD. The profound decrease in TEAC was associated with a decreased plasma protein sulfhydryl concentrations in acute exacerbations of COPD but not in smokers or in asthmatic subjects. Therefore smoking, acute exacerbations of COPD, and asthma are associated with a marked oxidant/antioxidant imbalance in the blood, associated with evidence of increased oxidative stress. The decreased antioxidant capacity in plasma may result from different mechanisms in these conditions.