Paenibacillus rhizoryzae sp. nov., isolated from rice rhizosphere.
Paenibacillus rhizoryzae sp. nov., isolated from rice rhizosphere.
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DOI：
10.1099/ijs.0.000376
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发表时间：
2015-09
影响因子：
2.8
通讯作者：
Lei Zhang;Ju-sheng Gao;Shuang Zhang;Rizwan Ali Sheirdil;Xiu-Cheng Wang;Xiao-Xia Zhang
中科院分区：
文献类型：
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作者：
Lei Zhang;Ju-sheng Gao;Shuang Zhang;Rizwan Ali Sheirdil;Xiu-Cheng Wang;Xiao-Xia Zhang
从湖南省水稻根际分离到一株革兰氏染色阳性、内孢子形成的杆状细菌，命名为103 -5（T）。根据表型特征和系统发育分析，该菌株被鉴定为类芽孢杆菌属的成员。16 SrRNA和rpoB基因序列与太湖类芽孢杆菌（PaenibacillustaihuensisCGMCC1.10966（T））同源性分别为97.2%和89.7%。10966（T）的DNA-DNA杂交值为33.4%。103 -5（T）的DNA G+C含量为47.5mol%。主要极性脂质为二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、未鉴定的氨基磷脂和未知磷脂。主要呼吸醌是MK-7。在细胞壁肽聚糖中发现的二氨基酸是内消旋二氨基庚二酸。主要的细胞脂肪酸是anteiso-C15：0、iso-C15：0和iso-C16：0。根据这些结果，113 -5（T）被认为是类芽孢杆菌属的一个新种，并建议将其命名为类芽孢杆菌根瘤亚种（Paenibacillus rhizobacillus sp. nov.）。模式菌株为103 -5（T）（= ACCC 19782（T）= DSM 29322（T））。
A Gram-stain-positive, endospore-forming, rod-shaped bacterium, designated 1ZS3-5(T), was isolated from rice rhizosphere in Hunan Province, PR China. The isolate was identified as a member of the genus Paenibacillus on the basis of phenotypic characteristics and phylogenetic inference analysis. The 16S rRNA and rpoB gene (β-subunit of bacterial RNA polymerase) sequences were closely related to those of Paenibacillus taihuensis CGMCC 1.10966(T) with similarities of 97.2% and 89.7%, respectively. The DNA-DNA hybridization value between 1ZS3-5(T) and P. taihuensis CGMCC 1.10966(T) was 33.4%. The DNA G+C content of 1ZS3-5(T) was 47.5 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, unidentified aminophospholipid and unknown phospholipid. The predominant respiratory quinone was MK-7. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0. Based on these results, 1ZS3-5(T) is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus rhizoryzae sp. nov. is proposed. The type strain is 1ZS3-5(T) ( = ACCC 19782(T) = DSM 29322(T)).