A high yield cellulose extraction system for small whole wood samples and dual measurement of carbon and oxygen stable isotopes
A high yield cellulose extraction system for small whole wood samples and dual measurement of carbon and oxygen stable isotopes
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DOI：
10.1016/j.chemgeo.2018.09.007
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发表时间：
2019-01-20
期刊：
影响因子：
3.9
通讯作者：
Helle, Gerhard
中科院分区：
文献类型：
--
作者：
Andreu-Hayles, Laia;Levesque, Mathieu;Helle, Gerhard
本文描述了从Lamont-Doherty地球天文台树木年轮实验室开发的小整木样品中提取a-纤维素的设备，并解释了化学提取和碳（δ C-13）和氧（δ O-18）稳定同位素双重分析的程序。在这里，我们提供了必要的步骤和指导方针，构建纤维素提取系统为少量的木材和树叶。该系统允许通过内部过滤管同时从150个样品中提取纤维素，其中用于纤维素提取的化学品被分批交换和消除。这种新的实现减少了处理时间，最大限度地减少了物理样品操作和潜在错误，增加了样品吞吐量，减少了化学品的数量和分析成本。我们还描述了在高温热解中使用高温转换元素分析仪（TC/EA）与Thermo delta V +质谱仪接口对树轮纤维素中δ C-13和δ O-18的双重测量。
This paper describes devices to extract a-cellulose from small whole wood samples developed at the Lamont-Doherty Earth Observatory Tree-Ring Lab and explains the procedures for chemical extractions and for the dual analysis of carbon (delta C-13) and oxygen (delta O-18) stable isotopes. Here, we provide the necessary steps and guidelines for constructing a cellulose extraction system for small amounts of wood and leaves. The system allows the simultaneous extraction of cellulose from 150 samples by means of in-house filter tubes, where chemicals used for the cellulose extraction are exchanged and eliminated in batches. This new implementation diminishes the processing time, minimizes physical sample manipulation and potential errors, increases sample throughput, and reduces the amount of chemicals and analytic costs. We also describe the dual measurement of delta C-13 and delta O-18 ratios in tree-ring cellulose using high-temperature pyrolysis in a High Temperature Conversion Elemental Analyzer (TC/EA) interfaced with a Thermo Delta V plus mass spectrometer.