Immunocytochemical Localization of NTPDases1 and 2 in the Neural Retina of Mouse and Zebrafish
Immunocytochemical Localization of NTPDases1 and 2 in the Neural Retina of Mouse and Zebrafish
复制标题
DOI：
10.1002/syn.20605
复制
发表时间：
2009-04-01
期刊：
影响因子：
2.3
通讯作者：
Paula Faillace, Maria
中科院分区：
文献类型：
--
作者：
Jimena Ricatti, Maria;Alfie, Lionel D.;Paula Faillace, Maria
三磷酸外核苷二磷酸水解酶（E-NTPDases）是一类水解细胞外二磷酸和三磷酸核苷的膜结合酶。已经提出E-NTPDases通过与特异性膜受体结合来控制介导细胞间通讯的细胞外核苷酸水平。在这里，我们显示了一个详细的免疫细胞化学定位的E-NTPDase家族的两种酶在视网膜层的两种脊椎动物物种，即小鼠和斑马鱼。在小鼠视网膜中，NTPDase 2主要定位于Muller胶质细胞和神经节细胞突起。NTPDase 1定位于神经元，因为它由水平和神经节细胞突起表达，这表明核苷酸如ATP和ADP可以在这些细胞的表面水解。在小鼠视网膜内血管中也检测到NTFDase1。关于斑马鱼，NTPDases1和2似乎是差异定位于水平细胞过程，感光节段，神经节细胞树突和轴突，但不存在穆勒神经胶质。此外，NTPDases1和2似乎是表达在含有增殖和分化细胞的斑马鱼视网膜的germinal边缘。视网膜匀浆从这两个物种表现出外ATP酶活性，这可能是由于至少NTPDases1和2，其表达在本报告中描述。我们的研究结果表明，视网膜层细胞外核苷酸/核苷浓度的分区调节，支持细胞外核苷酸介导的信号在脊椎动物视网膜中的相关作用。Synapse 63：291 - 307，2009年。(c)2008 Wiley-Liss，Inc.
Ectonucleoside triphosphate diphosphohydrolases (E-NTPDases) are a family of membrane-bound enzymes that hydrolyze extracellular di- and triphosphate nucleosides. E-NTPDases have been proposed to control extracellular nucleotide levels that mediate intercellular communication by binding to specific membrane receptors. Here we show a detailed immunocytochemical localization of two enzymes of the E-NTPDase family in the retinal layers of two vertebrate species, namely, the mouse and the zebrafish. In the mouse retina, NTPDase2 was chiefly localized in Muller glia and ganglion cell processes. NTPDase1 was located on neurons as we'll, since it was expressed by horizontal and ganglion cell processes, suggesting that nucleotides such as ATP and ADP can be hydrolyzed at the surface of these cells. NTFDase1 was also detected in intraretinal blood vessels of the mouse. Regarding zebrafish, NTPDases1 and 2 seem to be differentially localized in horizontal cell processes, photoreceptor segments, and ganglion cell dendrites and axons, but absent from Muller glia. Moreover, NTPDases1 and 2 appear to be expressed within the germinal margin of the zebrafish retina that contains proliferative and differentiating cells. Retinal homogenates from both species exhibited ecto-ATPase activity which might be attributed at least to NTPDases1 and 2, whose expression is described in this report. Our results suggest a compartmentalized regulation of extracellular nucleotide/nucleoside concentration in the retinal layers, supporting a relevant role for extracellular nucleotide mediated-signaling in vertebrate retinas. Synapse 63:291-307, 2009. (c) 2008 Wiley-Liss, Inc.