A novel biochip technology for detection of explosives -: TNT:: Synthesis, characterisation and application
A novel biochip technology for detection of explosives -: TNT:: Synthesis, characterisation and application
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DOI：
10.1016/j.snb.2005.07.025
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发表时间：
2006-02-27
影响因子：
8.4
通讯作者：
Liedberg, B
中科院分区：
文献类型：
--
作者：
Larsson, A;Angbrant, J;Liedberg, B
本文介绍了一种新的生物芯片技术的合成，表征和评价的爆炸性物质2，4，6-三硝基甲苯（TNT）的检测。两种类型的硫醇自组装产生的生物芯片上的黄金，即低聚（乙二醇）（OEG）-烷基硫醇封端的羟基和TNT-类似物（2，4-二硝基苯），分别。将三种不同的TNT类似物以各种比例与羟基封端的OEG-硫醇混合，以获得具有低非特异性结合的高选择性和灵敏度的生物芯片。生产的自组装单分子膜（SAM）的特点是彻底与零椭圆偏振，接触角测角，红外反射吸收光谱（IRAs）和X射线光电子能谱（XPS），他们都符合高标准的分子构象，包装和取向。该生物芯片，被设计为一个平台的竞争性无标记免疫分析和两个实时传感器表面等离子体共振（SPR）和石英晶体微天平（QCM）-用于监测的解离的在线固定化单克隆抗体产生的TNT。这三种TNT类似物都是开发功能性生物芯片的潜在候选者，尽管其中一种在抗体固定化后的较短恢复/稳定时间和较好的响应/负载容量比方面显示出上级特性。当在混合SAM中使用低抗原（TNT类似物）含量时，这是特别明显的。(c)2005 Elsevier B.V.保留所有权利。
This contribution describes the synthesis, characterisation and evaluation of a novel biochip technology for the detection of the explosive substance 2,4,6-trinitrotoluene (TNT). Two types of thiols are self-assembled to produce the biochip on gold, namely oligo(ethylene glycol) (OEG)-alkyl thiols terminated with a hydroxyl group and a TNT-analogue (2,4-dinitrobenzene), respectively. Three different TNT-analogues are mixed in various proportions with hydroxyl-terminated OEG-thiols to obtain highly selective and sensitive biochips with a low non-specific binding. The produced self-assembled monolayers (SAMs) are thoroughly characterised with null ellipsometry, contact angle goniometry, infrared reflection absorption spectroscopy (IRAs) and X-ray photoelectron spectroscopy (XPS) and they all meet high standards in terms of molecular conformation, packing and orientation. The biochip, is designed to function as a platform for a competitive label-free immunoassay and two real-time transducers-surface plasmon resonance (SPR) and quartz crystal microbalance (QCM)-are used to monitor the dissociation of on-line immobilised monoclonal antibodies produced against TNT. The three TNT-analogues are all potential candidates for the development of a functional biochip, though one of them displayed superior properties in terms of shorter recovery/stabilisation time after antibody immobilisation and a better response/loading capacity ratio. This is particularly evident when using low antigen (TNT-analogue) content in the mixed SAM. (c) 2005 Elsevier B.V. All rights reserved.