Surface immobilization of hexa-histidine-tagged adeno-associated viral vectors for localized gene delivery.
Surface immobilization of hexa-histidine-tagged adeno-associated viral vectors for localized gene delivery.
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作者：
腺相关病毒载体正在临床试验中进行广泛的探索，由于其安全性和递送效率，在治疗性基因递送方面具有重大前景。能够介导局部基因表达的基因传递技术可以通过减少目标区域外的扩散来进一步增强 AAV 在各种治疗应用中的潜力，从而减少脱靶副作用。我们通过基因工程改造了一种 AAV 变体，能够通过六组氨酸-金属结合相互作用以高亲和力与表面结合。这种固定化 AAV 载体系统可介导高效递送至接触表面的细胞，因此可能有望实现局部基因递送，这可能有助于 AAV 递送在基因治疗中的众多应用。
Adeno-associated viral vectors, which are undergoing broad exploration in clinical trials, have significant promise for therapeutic gene delivery due to their safety and delivery efficiency. Gene delivery technologies capable of mediating localized gene expression may further enhance AAV’s potential in a variety of therapeutic applications by reducing spread outside of a target region, which may thereby reduce off-target side effects. We have genetically engineered an AAV variant capable of binding to surfaces with high affinity via a hexahistidine-metal binding interaction. This immobilized AAV vector system mediates high efficiency delivery to cells that contact the surface and thus may have promise for localized gene delivery, which may aid numerous applications of AAV delivery to gene therapy.
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影响因子：
30.8
作者：
KAPLITT, MG;LEONE, P;DURING, MJ
通讯作者：
DURING, MJ
影响因子：
14.8
作者：
Koerber, James T.;Maheshri, Narendra;Schaffer, David V.
通讯作者：
Schaffer, David V.
DOI：
10.1073/pnas.94.13.6916
发表时间：
1997-06-24
影响因子：
11.1
作者：
Flannery, JG;Zolotukhin, S;Hauswirth, WW
通讯作者：
Hauswirth, WW
影响因子：
3.5
作者：
Mei, Lin;Jin, Xu;Levy, R. J.
通讯作者：
Levy, R. J.
影响因子：
14
作者：
Lei, Pedro;Padmashali, Roshan M.;Andreadis, Stelios T.
通讯作者：
Andreadis, Stelios T.