NeuroRhythmics: software for analyzing time-series measurements of saltatory movements in neuronal processes.
NeuroRhythmics: software for analyzing time-series measurements of saltatory movements in neuronal processes.
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NeuroRhythmics：用于分析神经元过程中跳跃运动的时间序列测量的软件。
DOI：
10.1016/j.jneumeth.2008.05.006
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发表时间：
2008
影响因子：
3
通讯作者：
Lindsley,TaraA
中科院分区：
文献类型：
--
作者：
Kerlin,AaronM;Lindsley,TaraA
活体神经元在体内和体外的延时成像显示，轴突和树突的生长是高度动态的，其特征在于伸展和收缩的交替周期。这些生长动力学与神经元发育的重要特征相关，并受到实验治疗的不同影响，但对潜在的细胞机制知之甚少。NeuroRhythm ®被开发用于半自动化特定的定量任务，这些任务涉及对表现出跳跃式延长的过程的二维时间序列图像的分析。该软件提供了关于生长期和非生长期的详细信息，该软件通过伸长的转变（即起始时间、平均速率、持续时间）和关于跳跃生长的总体模式的信息（即模式开始的时间、转变的频率、在生长状态与非生长状态下花费的相对时间）来识别。绘图和数值输出可轻松导入其他应用程序。用户可以选择指定用于识别生长行为转变的标准，这将软件的潜在应用扩展到不同类型或发育阶段的神经元以及表现出跳跃动力学的其他时间序列现象。NeuroRhythm ®将促进神经元中周期性轴突和树突生长的机制研究。
Time-lapse imaging of living neurons both in vivo and in vitro has revealed that the growth of axons and dendrites is highly dynamic and characterized by alternating periods of extension and retraction. These growth dynamics are associated with important features of neuronal development and are differentially affected by experimental treatments, but the underlying cellular mechanisms are poorly understood. NeuroRhythmics©was developed to semi-automate specific quantitative tasks involved in analysis of two-dimensional time-series images of processes that exhibit saltatory elongation. This software provides detailed information on periods of growth and nongrowth that it identifies by transitions in elongation (i.e. initiation time, average rate, duration) and information regarding the overall pattern of saltatory growth (i.e. time of pattern onset, frequency of transitions, relative time spent in a state of growth vs. nongrowth). Plots and numeric output are readily imported into other applications. The user has the option to specify criteria for identifying transitions in growth behavior, which extends the potential application of the software to neurons of different types or developmental stage and to other time-series phenomena that exhibit saltatory dynamics. NeuroRhythmics©will facilitate mechanistic studies of periodic axonal and dendritic growth in neurons.