Mechanical stretch-induced activation of skeletal muscle satellite cells is dependent on nitric oxide production in vitro.
Mechanical stretch-induced activation of skeletal muscle satellite cells is dependent on nitric oxide production in vitro.
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DOI：
10.1046/j.1344-3941.2002.00033.x
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发表时间：
2002-01-01
影响因子：
2
通讯作者：
Ito, T.
中科院分区：
文献类型：
--
作者：
Tatsumi, R.;Hattori, A.;Ito, T.
机械拉伸诱导培养的静止卫星细胞的活化，并且活化反应是由于肝细胞生长因子（HGF）从其与卫星细胞的细胞外缔合中快速释放以及其随后呈递给c-met受体。我们提供了新的证据，牵张激活是依赖于一氧化氮（NO）的生产。NO合成的竞争性抑制剂NG-硝基-L-精氨酸甲酯（L-NAME）的加入可以消除牵张激活，而活性较低的对映体NG-硝基-D-精氨酸甲酯盐酸盐则不能消除牵张激活。将HGF添加到L-NAME培养物中恢复了活化反应，表明L-NAME不直接抑制卫星细胞活化，而是在HGF释放的上游起作用。此外，卫星细胞裂解物的免疫印迹显示一氧化氮合酶的存在。这些实验表明，NO参与连接机械扰动卫星细胞的化学信号负责释放HGF从其隔离在体外。
Mechanical stretch induces activation of cultured quiescent satellite cells and the activation response is owing to rapid release of hepatocyte growth factor (HGF) from its extracellular association with satellite cells and its subsequent presentation to the c-met receptor. We provide new evidence that the stretch activation is dependent on nitric oxide (NO) production. Stretch activation could be abolished by the addition of NG-nitro-L-arginine methyl ester (L-NAME), a competitive inhibitor of NO synthesis, but not by NG-nitro-D-arginine methyl ester hydrochloride, a less active enantiomer of L-NAME. Adding HGF to the L-NAME culture restored the activation response, indicating that L-NAME does not directly inhibit satellite cell activation, but acts upstream from the HGF release. In addition, immunoblots of satellite cell lysate revealed the presence of nitric oxide synthase. These experiments suggest that NO is involved in linking mechanical perturbation of satellite cells to chemical signalling responsible for HGF release from its sequestration in vitro.