EFFECT OF DIETARY BENZYLSELENOCYANATE ON AZOXYMETHANE-INDUCED COLON CARCINOGENESIS IN MALE F344 RATS
EFFECT OF DIETARY BENZYLSELENOCYANATE ON AZOXYMETHANE-INDUCED COLON CARCINOGENESIS IN MALE F344 RATS
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DOI：
10.1080/01635589109514120
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发表时间：
1991-01-01
影响因子：
2.9
通讯作者：
REDDY, BS
中科院分区：
文献类型：
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作者：
NAYINI, JR;SUGIE, S;REDDY, BS
在雄性 F344 大鼠中研究了膳食硒氰酸苄酯 (BSC) 及其类似物、硫氰酸苄酯 (BTC) 和亚硒酸钠在氧化偶氮甲烷 (AOM) 诱导的肠道癌发生的起始和后阶段的影响。 打算用于初始研究的动物饲喂含有 25、50 和 100 ppm BSC（分别为 10、20 和 40 ppm 硒）以及 100 ppm BTC 和 4 ppm 硒（作为饮用水中的亚硒酸钠）的高脂肪（23.5% 玉米油）饮食；那些打算进行启动后研究的人喂食高脂肪对照饮食。 两周后，所有动物每周一次皮下注射AOM（15mg/kg体重），持续两周。 最后一次 AOM 注射三天后，将起始和起始后研究中的动物分别转移至高脂饮食和含有 BSC 和 BTC 以及饮用水中亚硒酸钠的高脂饮食。 该方案持续至 AOM 注射后 36 周。 在起始后阶段喂养 BSC 可以抑制小肠和结肠腺癌的发生率以及结肠腺癌的复数性。 亚硒酸钠仅在起始后阶段抑制结肠腺癌的发生率和多发性。 在起始阶段和起始后阶段喂食 BTC 没有抑制作用。 给予所有三种化合物，BSC (78%)、BTC (62%) 和亚硒酸钠 (44%)，结肠粘膜鸟氨酸脱羧酶活性均受到显着抑制。 结论：BSC对高脂饲料动物肠道癌的发生具有抑制作用。
The effect of dietary benzylselenocyanate (BSC) and its analogue, benzylthiocyanate (BTC), and sodium selenite during the initiation and postinitiation phases of azoxymethane (AOM)-induced intestinal carcinogenesis was studied in male F344 rats. Animals intended for initiation study were fed the high-fat (23.5% corn oil) diets containing 25, 50, and 100 ppm BSC (10, 20, and 40 ppm selenium, respectively) and 100 ppm BTC and 4 ppm selenium (as sodium selenite in drinking water); those intended for postinitiation study were fed the high-fat control diet. Two weeks later, all animals were injected subcutaneously with AOM (15 mg/kg body wt) once weekly for two weeks. Three days after the last AOM injection, animals in the initiation and postinitiation studies were transferred respectively to the high-fat diet and high-fat diets containing BSC and BTC and sodium selenite in drinking water. This regimen was continued until 36 weeks post-AOM injection. BSC inhibited the small intestinal and colon adenocarcinoma incidence and multiplicity of colon adenocarcinomas when fed during the postinitiation phase. Sodium selenite inhibited the incidence and multiplicity of colon adenocarcinomas only during the postinitiation phase. BTC had no inhibitory effect when fed during the initiation and postinitiation phases. The colonic mucosal ornithine decarboxylase activity was significantly inhibited by the administration of all three compounds, BSC (78%), BTC (62%), and sodium selenite (44%). It is concluded that the BSC has an inhibitory effect on the intestinal carcinogenesis in animals fed the high-fat diet.