Daily rhythms in Fos activity in the rat ventrolateral preoptic area and midline thalamic nuclei.
Daily rhythms in Fos activity in the rat ventrolateral preoptic area and midline thalamic nuclei.
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DOI：
10.1152/ajpregu.1998.275.5.r1620
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发表时间：
1998-11
期刊：
影响因子：
--
通讯作者：
C. Novak;A. Núñez
中科院分区：
文献类型：
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作者：
C. Novak;A. Núñez
本实验研究了在24小时的光-暗周期的大鼠大脑的睡眠和警觉相关的区域，包括腹外侧视前区（VLPO），丘脑室旁核（PVT），丘脑中央内侧核（CMT）的核磷蛋白Fos的表达。在Zeitgeber时间（ZT）1、ZT 5、ZT 12.5和ZT 17（ZT 0-ZT 12灯）灌注的大鼠脑切片上进行Fos（一种用作神经元活性指标的即刻早期基因产物）的免疫细胞化学。VLPO中Fos免疫阳性（Fos+）细胞的数量在ZT 5和12.5时升高（即，在周期的休息阶段期间或刚在周期的休息阶段之后）。在ZT 17和ZT 1，PVT和CMT中Fos+细胞数量增加，与VLPO呈180°异相。PVT和CMT中Fos+细胞数呈正相关，丘脑各核团Fos表达与VLPO中Fos+细胞数呈负相关。VLPO、PVT和CMT可以整合昼夜节律和稳态影响来调节睡眠-觉醒周期。
The present experiment investigated the expression of the nuclear phosphoprotein Fos over the 24-h light-dark cycle in regions of the rat brain related to sleep and vigilance, including the ventrolateral preoptic area (VLPO), the paraventricular thalamic nucleus (PVT), and the central medial thalamic nucleus (CMT). Immunocytochemistry for Fos, an immediate-early gene product used as an index of neuronal activity, was carried out on brain sections from rats perfused at zeitgeber time (ZT) 1, ZT 5, ZT 12.5, and ZT 17 (lights on ZT 0-ZT 12). The number of Fos-immunopositive (Fos+) cells in the VLPO was elevated at ZT 5 and 12.5 (i.e., during or just after the rest phase of the cycle). Fos+cell number increased at ZT 17 and ZT 1 in the PVT and CMT, 180° out of phase with the VLPO. A positive correlation was found between the numbers of Fos+ cells in the PVT and CMT, and Fos expression in each thalamic nucleus was negatively correlated with VLPO Fos+ cell number. The VLPO, PVT, and CMT may integrate circadian and homeostatic influences to regulate the sleep-wake cycle.