Development of a porcine excisional wound model
Development of a porcine excisional wound model
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DOI：
10.1197/s1069-6563(03)00339-7
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发表时间：
2003-10-01
影响因子：
4.4
通讯作者：
McClain, SA
中科院分区：
文献类型：
--
作者：
Singer, AJ;McClain, SA
目的：建立猪皮肤切向切除伤口模型。方法：这是对四只异氟烷麻醉的猪进行的前瞻性、纵向、盲法实验。使用设置在 300、600、750 或 900 mm 深度的电动皮刀创建 40 个标准化切除伤口（每个伤口 1 平方英寸）。用手术刀片创建全层伤口（每个深度 n = 8）。用聚氨酯薄膜处理伤口。 1、2、3、4、5 和 14 天后进行全层穿刺活检，以进行盲法组织病理学评估。主要结果是伤口再上皮化百分比 (REP)，通过将横截面中的新表皮长度除以样本直径计算得出（观察者间相关性 = 0.99）。方差分析用于比较伤口深度之间的 REP 百分比。这项研究有 80% 的功效检测到组间 REP 33% 的差异（双尾 alpha = 0.05）。结果：第 2-5 天，不同伤口深度的 REP 百分比存在显着差异（所有天均 p < 0.001）。较浅的伤口（300 至 600 mm）大多在第 4 天时重新上皮化，而深度范围为 750 至 900 mm 的伤口大多在第 5 天愈合。全层伤口即使在 14 天后也没有 REP 的证据。结论：作者描述了猪的切除伤口模型。伤口在三到五天内发生快速再上皮化
Objectives: To develop a porcine model for cutaneous tangential excisional wounds. Methods: This was a prospective, longitudinal, blinded experiment on four isoflurane-anesthetized swine. Forty standardized excisional wounds (I square inch each) were created with an electric dermatome set at a depth of 300, 600, 750, or 900 mum. Full-thickness wounds were created with a surgical blade (n = 8 for each depth). Wounds were treated with polyurethane film. Full-thickness punch biopsies were taken after one, two, three, four, five, and 14 days for blinded histopathological evaluation. The main outcome was the percent wound reepithelialization (REP) calculated by dividing the length of neoepidermis in cross-section by the specimen's diameter (Interobserver correlation = 0.99). Analysis of variance was used to compare percent REP among wound depths. This study had 80% power to detect a 33% difference in REP across groups (two-tailed alpha = 0.05). Results: There were significant differences in percent REP across wound depths for days 2-5 (p < 0.001 for all days). The more superficial wounds (300 to 600 mum) were mostly reepithelialized by day 4, whereas wounds ranging in depth from 750 to 900 mum were mostly healed by day 5. Full-thickness wounds had no evidence of REP even after 14 days. Conclusions: The authors describe an excisional wound model in swine. Rapid reepithelialization occurs within three to five days in wounds