Confocal analysis of primary cilia structure and colocalization with the Golgi apparatus in chondrocytes and aortic smooth muscle cells
Confocal analysis of primary cilia structure and colocalization with the Golgi apparatus in chondrocytes and aortic smooth muscle cells
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DOI：
10.1006/cbir.1997.0177
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发表时间：
1997-08-01
影响因子：
3.9
通讯作者：
Wheatley, DN
中科院分区：
文献类型：
--
作者：
Poole, CA;Jensen, CG;Wheatley, DN
脱酪氨酸和乙酰化的α-微管蛋白代表通常与真核细胞的中心体和初级纤毛的微管相关的微管蛋白的稳定库。虽然初级纤毛-中心体和中心体-高尔基体的关系已经被独立地确定，初级纤毛和高尔基体之间的精确结构关系尚未被具体定义。共聚焦免疫组化被用来定位脱酪氨酸（ID 5）和乙酰化（6-11B-1）微管蛋白抗体在初级纤毛的软骨细胞和平滑肌细胞，并证明其关系的高尔基复合体的互补凝集素染色与麦胚凝集素鉴定。结果表明，初级纤毛微管蛋白的分布和内在的结构变化，以及初级纤毛，高尔基体和核之间的解剖相互关系。我们认为，这些相互关系可能形成一个功能反馈机制，可以促进新合成的结缔组织大分子的定向分泌的一部分。(C)出版社：Academic Press Limited。
Detyrosinated and acetylated a-tubulins represent a stable pool of tubulin typically associated with microtubules of the centrosome and primary cilium of eukaryotic cells. Although primary cilium-centrosome and centrosome-Golgi relationships have been identified independently, the precise structural relationship between the primary cilium and Golgi has yet to be specifically defined. Confocal immunohistochemistry was used to localize detyrosinated (ID5) and acetylated (6-11B-1) tubulin antibodies in primary cilia of chondrocytes and smooth muscle cells, and to demonstrate their relationship to the Golgi complex identified by complementary lectin staining with wheat germ agglutinin. The results demonstrate the distribution and inherent structural variation of primary cilia tubulins, and the anatomical interrelationship between the primary cilium, the Golgi apparatus and the nucleus. We suggest that these interrelationships may form part of a functional feedback mechanism which could facilitate the directed secretion of newly synthesized connective tissue macromolecules. (C) 1997 Academic Press Limited.