Morphological and mechanical determinants of cellular uptake of deformable nanoparticles
Morphological and mechanical determinants of cellular uptake of deformable nanoparticles
复制标题
细胞摄取可变形纳米粒子的形态和机械决定因素
DOI：
10.1039/c8nr01521j
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发表时间：
2018-07-07
期刊：
影响因子：
6.7
通讯作者：
Liang, Haojun
中科院分区：
文献类型：
--
作者：
Chen, Liping;Li, Xuejin;Liang, Haojun
了解纳米粒子（NP）与细胞膜的相互作用并调节其细胞摄取过程对于设计具有最小毒性、高效率和长循环时间的药物递送系统至关重要。采用粗粒化过程，我们建立了具有可调形态和力学性能的弹性变形 NP 模型。我们发现，可变形纳米颗粒的细胞摄取取决于其形状：颗粒弹性的增加显着减慢了球形纳米颗粒的摄取速率，略微延迟了长形纳米颗粒的摄取速率，并促进了扁圆形纳米颗粒的摄取。通过分析内吞机制和自由能计算，仔细研究了其内在机制。这些发现为可变形纳米颗粒如何穿透细胞膜提供了独特的见解，并为设计有效的基于纳米颗粒的药物输送载体提供了新的可能性。
Understanding the interactions of nanoparticles (NPs) with cell membranes and regulating their cellular uptake processes are of fundamental importance to the design of drug delivery systems with minimum toxicity, high efficiency and long circulation time. Employing the procedure of coarse-graining, we built an elastically deformable NP model with tunable morphological and mechanical properties. We found that the cellular uptake of deformable NPs depends on their shape: an increase in the particle elasticity significantly slows the uptake rate of spherical NPs, slightly retards that of prolate NPs, and promotes the uptake of oblate NPs. The intrinsic mechanisms have been carefully investigated through analysis of the endocytic mechanisms and free energy calculations. These findings provide unique insights into how deformable NPs penetrate across cell membranes and offer novel possibilities for designing effective NP-based carriers for drug delivery.