In vivo measurement of the lineal density of red blood cells in human retinal capillaries using high-speed adaptive optics ophthalmoscopy.
In vivo measurement of the lineal density of red blood cells in human retinal capillaries using high-speed adaptive optics ophthalmoscopy.
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DOI：
10.1364/ol.428538
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发表时间：
2021-07-15
期刊：
影响因子：
3.6
通讯作者：
Zhang, Yuhua
中科院分区：
文献类型：
--
作者：
Gu, Boyu;Sarraf, David;Ip, Michael;Sadda, Srinivas R.;Zhang, Yuhua
我们提出了一种自动化的方法来测量红细胞（RBC）的线密度在人类视网膜毛细血管使用自适应光学近共焦检眼镜（AONCO）。从AONCO图像中提取红细胞在视网膜毛细血管中流动的时空轨迹，使用Gabor滤波器、血管性滤波器和Hough变换进行增强。对12名受试者的12只眼的43个毛细血管段（每个50 μm长）进行了分析，与手工计数结果相比，自动化方法的测量误差为0.09个细胞。我们的方法提供了一个工具，用于评估红细胞在视网膜毛细血管中的空间分布。
We present an automated method for measuring the lineal density of red blood cells (RBCs) in human retinal capillaries using adaptive optics near-confocal ophthalmoscopy (AONCO). The spatiotemporal traces of RBCs flowing in retinal capillaries were extracted from AONCO images, enhanced using the Gabor filter, the vesselness filter, and the Hough transform. A total of 43 capillary segments (each 50 μm long) were analyzed in 12 eyes of 12 subjects and the measurement error of the automated method was 0.09 cell over 50 μm compared with results obtained by manual counting. Our method provides a tool for assessing RBCs spatial distribution in retinal capillaries.