Gene expression in chorionic villous samples at 11 weeks of gestation in women who develop pre-eclampsia later in pregnancy: implications for screening
Gene expression in chorionic villous samples at 11 weeks of gestation in women who develop pre-eclampsia later in pregnancy: implications for screening
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DOI：
10.1002/pd.2675
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发表时间：
2011-02-01
影响因子：
3
通讯作者：
Rizzo, Nicola
中科院分区：
文献类型：
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作者：
Farina, Antonio;Zucchini, Cinzia;Rizzo, Nicola
目的探讨子痫前期患者绒毛组织(CVSS)基因表达谱。方法病例对照研究包括5例子痫前期患者和30例正常对照。我们定量检测了正常人和PE患者CVS组织标本上的mRNA表达。检测组织蛋白酶(CTSD)、血管生成素2(ANGPT2)、白介素8、趋化因子(C-X-C基序)配体10、神经激动素B(NKB)、基质金属肽酶9、主要组织相容性复合体I、C类(HLAC)和人类白细胞抗原G(HLAG)的mRNA表达。结果与对照组相比，除CTSD和ANGPT2外，PE组所有基因的平均观察等级均发生了显著变化。与正常对照组相比，NKB和HLAC基因的表达异常程度最高。结论本研究直接表明滋养层细胞侵袭或子宫胎盘血流动力学适应相关基因的表达在早孕滋养层细胞中发生了改变。这些结果表明残存CVS可作为PE的一种可能的筛查方法。版权所有(C)2011 John Wiley&Sons，Ltd.
Objectives To determine the gene expression profile in chorionic villous samples (CVSs) of women destined to develop pre-eclampsia (PE).Method Case-control study encompassing five women destined to develop PE [cases matched for gestational age with 30 controls]. We quantified mRNA expression on tissue samples from CVS of normal and PE patients. We then assessed mRNA expressions of cathepsin (CTSD), angiopoietin 2 (ANGPT2), interleukin 8, chemokine (C-X-C motif) ligand 10, neurokinin B (NKB), matrix metallopeptidase 9, major histocompatibility complex, class I, C (HLA-C)and human leukocyte antigen-G (HLA-G). Data were analyzed by nonpararnetric rank analysis.Results For all the mRNA species considered in this study, except CTSD and ANGPT2, all the mean observed ranks in the PE group were significantly altered compared with the rank expectation among controls. mRNA for NKB and HLA-C were the markers with the highest degree of aberration in PE, compared with those in controls.Conclusion Our study has directly showed that gene expressions relating to trophoblastic cell invasion or utero-placental hemodynamic adaptation are altered in the first trimester trophoblasts that go on to develop PE later. These results posit the use of residual CVS as a possible screening method for PE. Copyright (C) 2011 John Wiley & Sons, Ltd.