Dynamic DNA Structures
Dynamic DNA Structures
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动态DNA结构
DOI：
10.1002/smll.201900228
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发表时间：
2019
期刊：
影响因子：
13.3
通讯作者：
Ke Yonggang
中科院分区：
文献类型：
--
作者：
Zhang Yingwei;Pan Victor;Li Xue;Yang Xueqin;Li Haofei;Wang Pengfei;Ke Yonggang
动态DNA结构是一种使用可编程DNA自组装构建的DNA结构，具有响应环境刺激重新配置其构象的能力。设计动态DNA结构的一般策略是将可重构元件集成到常规静态DNA结构中，所述常规静态DNA结构可以由包括DNA折纸和DNA瓦片的各种方法组装。常用的可重构元件的范围从链置换反应、特殊结构基序、靶结合DNA适体和碱基堆积组分到DNA构象变化结构域等。动态DNA结构的形态学变化可以通过成像技术可视化，或者可以翻译为其他可检测的读出信号（例如，荧光）。由于其可编程的识别环境线索的能力具有高度的特异性，动态DNA结构体现了强大的和多功能的系统，在传感和成像生物分析物，在提供分子货物，并在建设可编程系统，能够进行复杂的任务有很大的希望的缩影。
Dynamic DNA structures, a type of DNA construct built using programmable DNA self‐assembly, have the capability to reconfigure their conformations in response to environmental stimulation. A general strategy to design dynamic DNA structures is to integrate reconfigurable elements into conventional static DNA structures that may be assembled from a variety of methods including DNA origami and DNA tiles. Commonly used reconfigurable elements range from strand displacement reactions, special structural motifs, target‐binding DNA aptamers, and base stacking components, to DNA conformational change domains, etc. Morphological changes of dynamic DNA structures may be visualized by imaging techniques or may be translated to other detectable readout signals (e.g., fluorescence). Owing to their programmable capability of recognizing environmental cues with high specificity, dynamic DNA structures embody the epitome of robust and versatile systems that hold great promise in sensing and imaging biological analytes, in delivering molecular cargos, and in building programmable systems that are able to conduct sophisticated tasks.