Histamine production by human neutrophils
Histamine production by human neutrophils
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DOI：
10.1096/fj.12-223867
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发表时间：
2013-07-01
期刊：
影响因子：
4.8
通讯作者：
Monteseirin, Javier
中科院分区：
文献类型：
--
作者：
Alcaniz, Lorena;Vega, Antonio;Monteseirin, Javier
组胺是过敏反应发生的重要介质。只有一小部分人类细胞类型能够产生组胺。之前没有研究表明人类中性粒细胞也在其中。目前的工作是分析人类中性粒细胞是否产生组胺，并确定哪些激动剂参与人类中性粒细胞产生组胺。通过定量PCR、蛋白质印迹和流式细胞术分析确定人中性粒细胞中组氨酸脱羧酶的表达。该酶的活性通过 ELISA 测定，该酶测量用一组经典激动剂刺激的中性粒细胞培养物上清液中的组胺。人类中性粒细胞是真正的产生组胺的细胞。中性粒细胞储存约 0.29 pg/细胞，并以抗原依赖性方式和在其他中性粒细胞激动剂刺激下释放约 50% 的组胺含量。组氨酸脱羧酶（组胺产生的限速酶）在过敏患者的中性粒细胞中的基础表达高于健康供体的中性粒细胞。由于多种原因，我们的结果不能归因于细胞污染。 LPS 不能诱导嗜碱性粒细胞释放组胺，但可以诱导中性粒细胞释放组胺；我们在中性粒细胞制剂中没有检测到嗜碱性粒细胞、单核细胞或淋巴细胞。尽管检测到嗜酸性粒细胞，但其仅占最终细胞群的 0.001-0.004%，并且它们在抗原或 LPS 刺激下不储存或释放组胺。过敏患者所过敏的抗原会刺激中性粒细胞释放组胺。这些观察结果代表了中性粒细胞作为过敏性疾病中组胺可能来源的新观点。Alcaniz, L.、Vega, A.、Chacon, P.、El Bekay, R.、Ventura, I.、Aroca, R.、Blanca, M.、Bergstralh, D. T.、Monteseirin, J.人类中性粒细胞产生组胺。
Histamine is an important mediator in the development of allergic reactions. Only a small subset of human cell types is able to produce histamine. No previous studies have shown that human neutrophils are among them. The present work was undertaken to analyze whether human neutrophils produce histamine, and to determine what agonists are involved in histamine production by human neutrophils. The expression of histidine decarboxylase in human neutrophils was established by quantitative PCR, Western blotting, and flow cytometry analysis. The activity of the enzyme was determined by ELISA, which measured histamine in the culture supernatant of neutrophils stimulated with a set of classical agonists. Human neutrophils are bona fide histamine-producing cells. Neutrophils store approximate to 0.29 pg/cell and release approximate to 50% of the histamine content in an antigen-dependent manner and on stimulation with other neutrophil agonists. Basal expression of histidine decarboxylase, the rate-limiting enzyme in histamine production, is higher in neutrophils from patients with allergies than from healthy donors. Our results cannot be ascribed to cell contamination for several reasons. LPS failed to induce histamine release by basophils, whereas it induced histamine release by neutrophils; and we did not detect basophils, monocytes, or lymphocytes in our neutrophil preparations. Eosinophils, albeit detected, were only 0.001-0.004% of the final cell population, and they did not store or release histamine on antigen or LPS stimulation. Antigens to which patients with allergies were sensitized stimulated release of histamine from neutrophils. These observations represent a novel view of neutrophils as possible source of histamine in the allergic diseases.Alcaniz, L., Vega, A., Chacon, P., El Bekay, R., Ventura, I., Aroca, R., Blanca, M., Bergstralh, D. T., Monteseirin, J. Histamine production by human neutrophils.