The identification of c-Kit-positive cells in the intestine of chicken
The identification of c-Kit-positive cells in the intestine of chicken
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DOI：
10.3382/ps.2011-02076
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发表时间：
2012-09-01
期刊：
影响因子：
4.4
通讯作者：
Chen, Q.
中科院分区：
文献类型：
--
作者：
Yang, P.;Yu, Z.;Chen, Q.
鸟类Cajal间质细胞(ICC)的超微结构已被研究，但由于缺乏合适的标记，这些细胞的分布尚不清楚。本研究采用原位杂交组织化学方法和实时定量聚合酶链式反应技术，对鸡肠道中c-Kit阳性细胞进行了鉴定和表达。C-Kit mRNA染色阳性的细胞有两种。第一组由被鉴定为ICC的纺锤形或双极细胞组成。ICC分布于环行肌和纵行肌之间的肌间神经丛水平，与环行肌和纵行肌肌束内的平滑肌细胞混杂在一起。在粘膜下层也可见ICC。第二组由圆形细胞组成，类似于肥大细胞。肥大细胞主要分布在固有层区和粘膜下层。C-Kit基因的实时定量聚合酶链式反应显示c-Kit基因在整个肠段的肌层和粘膜均有表达，但不同部位c-Kit的表达不同。本研究首次确凿地揭示了ICC在成年鸡肠道中的分布，定量了c-Kit mRNA在成年鸡肠道中的表达，并对c-Kit阳性细胞类型进行了形态比较。
The ultrastructure of the interstitial cells of Cajal (ICC) has been examined in birds, but the distribution of these cells remains obscure because a suitable marker is lacking. In the present study, the identification and expression of c-Kit-positive cells in the chicken intestine were demonstrated by means of in situ hybridization histochemistry and the expression of the c-Kit gene by real-time quantitative PCR. Two types of cells stained positive for c-Kit mRNA. The first group consisted of spindle-shaped or bipolar cells identified as ICC. The ICC were found at a variety of locations: at the level of the myenteric plexus between the circular and longitudinal muscle and intermingled with smooth muscle cells within muscle bundles in the circular and longitudinal muscle layers. The ICC were also identified along the submucosal layer. The second group was composed of round-shaped cells, which resembled mast cells. Mast cells were mainly found in the lamina propria region as well as in the submucosal layer. The expression of the c-Kit gene by real-time quantitative PCR revealed the expression of c-Kit mRNA throughout the lamina muscularis and mucosa of the intestine; however, the quantitation was variable in different regions. This study reveals conclusively for the first time the distribution of ICC, quantifies the expression of c-Kit mRNA in the intestine of adult chicken, and also compares the c-Kit-positive cell types morphologically.