ACELLULAR DENTAL MATRICES PROMOTE FUNCTIONAL-DIFFERENTIATION OF AMELOBLASTS
ACELLULAR DENTAL MATRICES PROMOTE FUNCTIONAL-DIFFERENTIATION OF AMELOBLASTS
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DOI：
10.1111/j.1432-0436.1985.tb00311.x
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
RUCH, JV
中科院分区：
文献类型：
--
作者：
KARCHERDJURICIC, V;STAUBLI, A;RUCH, JV
EDTA处理的出生后的小鼠磨牙的无细胞牙基质组成的基底层，前牙本质，牙本质和釉质的隔离。将胰蛋白酶分离的胚胎小鼠下颌磨牙牙乳头和成釉器官与分离的基质结合并进行体外培养。在这种重组中，从未观察到功能性成牙本质细胞。与咬合前牙本质上皮面接触的成釉细胞明显分化。胍-EDTA或乙酸处理的基质不能促进成釉细胞的功能分化。这些数据进行了讨论的上皮间充质的相互作用参与牙发育。
EDTA treatment of post-natal mouse molars made possible the isolation of cell-free dental matrices composed of basal lamina, predentin, dentin and enamel. Trypsin-isolated dental papillae and enamel organs from embryonic-mouse mandibular molars were combined with isolated matrices and cultured in vitro. In such recombinations, functional odontoblasts were never observed. Competent preameloblasts in contact with the epithelial side of occlusal predentin overtly differentiated. Matrices treated with guanidine-EDTA or acetic acid were unable to promote the functional differentiation of ameloblasts. These data are discussed in terms of the epitheliomesenchymal interactions involved in odontogenesis.