Apolipoprotein 4 may increase viral load and seizure frequency in mesial temporal lobe epilepsy patients with positive human herpes virus 6B
Apolipoprotein 4 may increase viral load and seizure frequency in mesial temporal lobe epilepsy patients with positive human herpes virus 6B
复制标题
载脂蛋白 4 可能会增加人类疱疹病毒 6B 阳性的内侧颞叶癫痫患者的病毒载量和癫痫发作频率
DOI：
10.1016/j.neulet.2014.12.063
复制
发表时间：
2015-04-23
影响因子：
2.5
通讯作者：
Li, Jin-Mei
中科院分区：
文献类型：
--
作者：
Huang, Cheng;Yan, Bo;Li, Jin-Mei
本研究旨在探讨载脂蛋白4（ApoE 4）是否与内侧颞叶癫痫（MTLE）中人类疱疹病毒（HHV）-6B的存在相关。应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测46例MTLE患者和19例对照组的ApoE基因多态性。应用巢式PCR和实时荧光PCR检测HHV-6 B DNA，免疫组化检测HHV-6 B蛋白。与HHV-6 B（+）/非ApoE 4患者相比，HHV-6 B（+）/ApoE 4 MTLE患者的病毒DNA载量显著增加（p = 0.031）。免疫组化半定量分析显示，HHV-6 B（+）/ApoE 4患者中HHV-6 B蛋白G116/64/54、P98和U94阳性细胞数显著高于HHV-6 B（+）/非ApoE 4患者（p分别为0.009、0.035和0.009）。HHV-6 B（+）/ApoE 4患者的癫痫发作频率高于HHV-6 B（+）/非ApoE 4患者（p = 0.005）。MTLE伴和不伴HHV-6 B之间ApoE等位基因无显著差异（p = 0.115）。ApoE 4与MTLE中HHV-6 B的初始感染无关。而ApoE 4可能促进HHV-6 B再激活、DNA复制、病毒蛋白表达，并增加MTLE的发作频率。需要进一步研究以了解ApoE和HHV-6 B之间相互作用的生物分子机制。(C)2015爱思唯尔爱尔兰有限公司版权所有。
This study investigated whether apolipoprotein 4 (ApoE4) was associated with the presence of human herpes virus (HHV)-6B in mesial temporal lobe epilepsy (MTLE). Polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP) was used to determine ApoE polymorphism in 46 patients with MTLE and 19 controls. Nested PCR and real-time PCR were applied to determine HHV-6B DNA and immunohistochemistry (IHC) for HHV-6B protein. Viral DNA load was significantly increased in MTLE patients with HHV-6B(+)/ApoE4 compared with those with HHV-6B(+)/non-ApoE4 (p = 0.031). Semi-quantitative analysis of IHC showed significantly increased number of positive cells for HHV-6B proteins G116/64/54, P98 and U94 in patients with HHV-6B(+)/ApoE4 than HHV-6B(+)/non-ApoE4 (p = 0.009, 0.035 and 0.009, respectively). Patients with HHV-6B(+)/ApoE4 showed higher seizure frequency than those with HHV-6B(+)/non-ApoE4 (p = 0.005). There was no significant difference of ApoE alleles between MTLE with and without HHV-6B (p = 0.115). ApoE4 was not associated with initial infection of HHV-6B in MTLE. However, ApoE4 may facilitate HHV-6B reactivation, DNA replication, virus protein expression and increase seizure frequency in MTLE. Further investigations are needed to understand the biomolecular mechanism underlying interaction between ApoE and HHV-6B. (C) 2015 Elsevier Ireland Ltd. All rights reserved.