BEHAVIOUR OF HORSERADISH PEROXIDASE IN AOT REVERSED MICELLES
BEHAVIOUR OF HORSERADISH PEROXIDASE IN AOT REVERSED MICELLES
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辣根过氧化物酶在 AOT 反胶束中的行为
DOI：
10.3109/10242420109105265
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发表时间：
2001
影响因子：
1.8
通讯作者：
D. Prazeres
中科院分区：
文献类型：
--
作者：
A. Azevedo;L. Fonseca;D. L. Graham;J. Cabral;D. Prazeres
以愈创木酚（2-甲氧基苯酚）为电子给体，研究了辣根过氧化物酶（HRP）在异辛烷和十氢萘中的AOT反胶团中的增溶活性和稳定性。在这两个反胶束系统中的酶的活性随着水含量的增加，直到达到一个最大值，保持相当恒定的水含量高于3.05%（v/v）的异辛烷和2.20%的十氢萘。在AOT/异辛烷体系中研究了pH对催化剂活性分布的影响。该酶在pH 7和pH 8下对高于3.05%（v/v）的水含量是完全活性的，但在pH 9下完全失活。还研究了表面活性剂浓度对HRP活性的影响。在低含水量下，观察到强烈的依赖性，而当含水量值高于2.7%（v/v）时，没有观察到进一步的活性增加。HRP的稳定性被认为是强烈依赖于系统的水含量与更高的水含量值获得更高的稳定性水平。HRP的稳定性也受到底物存在的影响。虽然当酶与愈创木酚一起孵育时稳定性显著增加，但在所研究的浓度下，它似乎不会受到过氧化氢存在的强烈影响。
The activity and stability of horseradish peroxidase (HRP) solubilised in AOT reversed micelles in isooctane and decalin was studied using guaiacol (2-methoxyphenol) as the electron donor. The activity of the enzyme in both reversed micellar systems increases with the water content until reaching a maximum value that remains fairly constant for water contents higher than 3.05% (v/v) in isooctane and 2.20% in decalin. The effect of pH on the activity profile was studied in the system AOT/isooctane. The enzyme is fully active at pH 7 and 8 for water contents higher than 3.05% (v/v) but it was completely deactivated at pH 9. The effect of surfactant concentration on HRP activity was also investigated. At low water contents a strong dependence was observed, whilst no further activity increase was observed for water content values higher than 2.7% (v/v). The stability of HRP was found to be strongly dependent on the water content of the system with higher levels of stability obtained for higher values of water content. HRP stability is also affected by the presence of substrates. Whilst the stability increases markedly when the enzyme is incubated with guaiacol, it does not appear to be so strongly affected by the presence of hydrogen peroxide, at the concentrations studied.
过氧化物酶愈创木酚测定中出现意外的副反应。
影响因子：
2.9
作者：
Taurog,A;Dorris,ML;GuziecJr,FS
通讯作者：
GuziecJr,FS