Genome-wide transcriptomic analysis of a superior biomass-degrading strain of A. fumigatus revealed active lignocellulose-degrading genes.
Genome-wide transcriptomic analysis of a superior biomass-degrading strain of A. fumigatus revealed active lignocellulose-degrading genes.
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DOI：
10.1186/s12864-015-1658-2
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发表时间：
2015-06-16
期刊：
影响因子：
4.4
通讯作者：
Zhang R
中科院分区：
文献类型：
--
作者：
Miao Y;Liu D;Li G;Li P;Xu Y;Shen Q;Zhang R
木质纤维素是地球上最丰富的天然物质之一，各种腐营养微生物，特别是丝状真菌，可以有效地降解木质纤维素。它由复杂的碳水化合物和芳香族聚合物组成，存在于植物细胞壁和植物碎片中。烟曲霉Z5是从堆肥堆中分离到的一株具有高效植物生物质降解能力的菌株。对Z5的2900万个碱基对的基因组进行了测序，预测并注释了9540个蛋白质编码基因。基因组分析揭示了一个令人印象深刻的基因编码纤维素酶，半纤维素酶和果胶酶参与木质纤维素生物质降解。A.比较了蔗糖、燕麦木聚糖、Avicel PH-101和稻草对烟曲霉Z5的诱导效果。与蔗糖对照相比，木聚糖、纤维素和稻草中分别有444、1711和1386个基因表达差异显著。基因组和转录组数据的结合分析提供了对A.烟熏。该研究为进一步分析在多糖底物存在下被高度诱导的基因提供了基础，也为生物质降解和异源蛋白表达提供了有用的信息。本文的在线版本（doi：10.1186/s12864 - 015 - 1658 - 2）包含补充材料，可供授权用户使用。
Various saprotrophic microorganisms, especially filamentous fungi, can efficiently degrade lignocellulose that is one of the most abundant natural materials on earth. It consists of complex carbohydrates and aromatic polymers found in the plant cell wall and thus in plant debris. Aspergillus fumigatus Z5 was isolated from compost heaps and showed highly efficient plant biomass-degradation capability. The 29-million base-pair genome of Z5 was sequenced and 9540 protein-coding genes were predicted and annotated. Genome analysis revealed an impressive array of genes encoding cellulases, hemicellulases and pectinases involved in lignocellulosic biomass degradation. Transcriptional responses of A. fumigatus Z5 induced by sucrose, oat spelt xylan, Avicel PH-101 and rice straw were compared. There were 444, 1711 and 1386 significantly differently expressed genes in xylan, cellulose and rice straw, respectively, when compared to sucrose as a control condition. Combined analysis of the genomic and transcriptomic data provides a comprehensive understanding of the responding mechanisms to the most abundant natural polysaccharides in A. fumigatus. This study provides a basis for further analysis of genes shown to be highly induced in the presence of polysaccharide substrates and also the information which could prove useful for biomass degradation and heterologous protein expression. The online version of this article (doi:10.1186/s12864-015-1658-2) contains supplementary material, which is available to authorized users.