Human CD4+ T cells are predominantly distributed among six phenotypically and functionally distinct subsets
Human CD4+ T cells are predominantly distributed among six phenotypically and functionally distinct subsets
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DOI：
10.4049/jimmunol.175.9.5765
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发表时间：
2005-11-01
影响因子：
4.4
通讯作者：
Callan, MFC
中科院分区：
文献类型：
--
作者：
Amyes, E;McMichael, AJ;Callan, MFC
人类 T 细胞是异质的，其表型、功能能力和 Ag 接触史各不相同。用于分类CD4(+) T细胞的功能相关模型的推导受到可使用经典四色流式细胞术测量的参数数量的限制。在本研究中，我们利用五色流式细胞术试剂的引入，开发了一种详细的、具有功能意义的方案，用于对人类 CD4(+) T 细胞进行分类。我们发现CD4(+) T细胞主要分布在八个可能的区室中的六个中，通过CCR7、CD45RA和CD28的表达来识别。我们证明了新的表型和功能相关性，证明选择这三种分子来定义 CD4(+) T 细胞区室是合理的。我们注意到具有不同 Ag 特异性的 CD4(+) T 细胞在所描述的六个亚群中分布不同。根据这些结果，我们提出了一个用于外周 CD4(+) T 细胞分类的横截面模型。了解 T 细胞在该模型上的位置可以了解其功能能力，并可以反映其接触 Ag 的历史。
Human T cells are heterogeneous, varying in terms of their phenotype, functional capabilities, and history of Ag encounter. The derivation of a functionally relevant model for classifying CD4(+) T cells has been hampered by limitations on the numbers of parameters that may be measured using classical four-color flow cytometry. In this study we have taken advantage of the introduction of reagents for five-color flow cytometry to develop a detailed, functionally meaningful scheme for classifying human CD4(+) T cells. We show that CD4(+) T cells are predominantly distributed among six of eight possible compartments, identified by the expression of CCR7, CD45RA, and CD28. We demonstrate novel phenotypic and functional correlates that justify the choice of these three molecules to define CD4(+) T cell compartments. We note that CD4(+) T cells with different Ag specificities are distributed differently among the six described subsets. On the basis of these results, we propose a cross-sectional model for classification of peripheral CD4(+) T cells. Knowledge of where T cells lie on this model informs about their functional capacity and can reflect their history of Ag exposure.