Nucleotide sequence of the F0 subunits of the sodium dependent F1F0 ATPase of Propionigenium modestum.
Nucleotide sequence of the F0 subunits of the sodium dependent F1F0 ATPase of Propionigenium modestum.
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Propionigeniummodem 钠依赖性 F1F0 ATP 酶 F0 亚基的核苷酸序列。
DOI：
10.1093/nar/18.19.5887
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发表时间：
1990
影响因子：
14.9
通讯作者：
R. D. Simoni
中科院分区：
文献类型：
--
作者：
U. Esser;L. Krumholz;R. D. Simoni
Propionigenium modestum是一种严格厌氧的革兰氏阴性细菌，其从琥珀酸发酵为丙酸和CO2。甲基丙二酰辅酶A脱羧过程中产生的能量用于产生跨细胞质膜的钠离子梯度。然后，钠梯度的能量驱动由钠依赖性FIFO ATP酶催化的ATP合成。已显示P. modestum的ATP酶将钠离子或质子的移位与ATP水解偶联（1）。这种酶是有趣的，因为其他表征的细菌FIFO ATP酶被认为是严格的质子易位。编码ATP酶多肽的大肠杆菌unc操纵子由编码该酶的F1和Fo结构域的9个基因组成。Fo部分是膜固有的，由E. coli unc操纵子。来自P. modestun的Fo亚基已被测序，序列如图所示。这些亚基被称为uncB、uncE和uncF，它们的阅读框开始分别在下面所示的P. modestum核苷酸序列中的碱基编号235、1168和1545处开始。Shine-Dalgamo序列以及各个亚基的终止密码子加下划线。起始密码子以粗体字母显示。亚基B（由uncF编码）的翻译起始可能起始于巨大芽孢杆菌中uncB报告的UUG起始密码子，或起始于位于同一阅读框3'端15个核苷酸处的AUG。在用染色体P. modestum DNA构建噬菌体X Dash文库后获得该序列。然后用寡核苷酸筛选文库。
Propionigenium modestum is a strictly anaerobic gram negative bacterium which grows from the fermentation of succinate to propionate and CO2. The energy generated during the decarboxylation of methyl-malonyl CoA is used to generate a sodium ion gradient across the cytoplasmic membrane. The energy of the sodium gradient then drives ATP synthesis catalyzed by a sodium dependent FIFO ATPase. The ATPase of P. modestum has been shown to couple translocation of either sodium ions or protons to ATP hydrolysis (1). This enzyme is interesting since other characterized bacterial FIFO ATPases are thought to be strictly proton translocating. The Escherichia coli unc operon, which codes for the ATPase polypeptides, consists of nine genes coding for the F1 and Fo domains of the enzyme. The Fo portion is membrane intrinsic and is encoded by three genes organized in a linear fashion in the E. coli unc operon. The Fo subunits from P. modestun have been sequenced and the sequence is shown here. The subunits are termed uncB, uncE and uncF and their reading frames begin at base numbers 235, 1168 and 1545 respectively in the P. modestum nucleotide sequence shown below. The Shine-Dalgamo sequences as well as the stop codons for the individual subunits are underlined. The start codons appear in bold letters. Translation initiation for subunit b (coded by uncF) probably starts with a UUG start codon as reported for uncB in Bacillus megaterium or with the AUG located 15 nucleotides further 3' in the same reading frame. The sequence was obtained after construction of a bacteriophage X Dash library with chromosomal P. modestum DNA. The library was then screened with an oligonucleotide