Crystal structure of the PX domain of Vps17p from Saccharomyces cerevisiae.
Crystal structure of the PX domain of Vps17p from Saccharomyces cerevisiae.
复制标题
酿酒酵母 Vps17p PX 结构域的晶体结构。
DOI：
10.1107/s2053230x22004472
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Maita N.
中科院分区：
文献类型：
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作者：
Obita T;Inaka K;Kohda D;Maita N.
的PX（phox同源）结构域的酿酒酵母Vps 17 p蛋白的结构测定提出了一个具有挑战性的情况下，分子置换，因为它具有非晶体学对称性接近结晶轴。在国际空间站微重力条件下生长的衍射质量晶体与AlphaFold 2预测的高度准确的模板结构相结合，为成功确定晶体结构提供了关键。虽然在许多PX结构域中看到了Vps 17 p PX结构域的结构，但在典型的磷脂酰肌醇磷酸（PtdIns-P）结合位点周围没有发现碱性残基，表明不能结合PtdIns-P分子。
The structure determination of the PX (phox homology) domain of the Saccharomyces cerevisiae Vps17p protein presented a challenging case for molecular replacement because it has noncrystallographic symmetry close to a crystallographic axis. The combination of diffraction-quality crystals grown under microgravity on the International Space Station and a highly accurate template structure predicted by AlphaFold2 provided the key to successful crystal structure determination. Although the structure of the Vps17p PX domain is seen in many PX domains, no basic residues are found around the canonical phosphatidylinositol phosphate (PtdIns-P) binding site, suggesting an inability to bind PtdIns-P molecules.