Molecular Characterization and Pathogenicity of Infectious Bronchitis Coronaviruses: Complicated Evolution and Epidemiology in China Caused by Cocirculation of Multiple Types of Infectious Bronchitis Coronaviruses
Molecular Characterization and Pathogenicity of Infectious Bronchitis Coronaviruses: Complicated Evolution and Epidemiology in China Caused by Cocirculation of Multiple Types of Infectious Bronchitis Coronaviruses
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DOI：
10.1159/000227134
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发表时间：
2009-01-01
期刊：
影响因子：
4.6
通讯作者：
Kong, Xiangang
中科院分区：
文献类型：
--
作者：
Liu, Shengwang;Zhang, Xiaonan;Kong, Xiangang
目的：监测和研究近年来中国传染性支气管炎病毒（ibv）的分子流行病学、进化和致病性，进一步了解ibv的进化。方法：从国内商品鸡中分离到37株IBV。对分离株进行RT-PCR、测序、分型和全基因分析。另外，选取4株IBV分离株，对特定的无致病性鸡进行实验感染，研究其致病性。结果：三种类型的IBV近年来在中国鸡群中共流行。在不同类型的IBV中发现了S1蛋白区域的独特插入和缺失。此外，从一只蛋鸡中分离鉴定出一株新的IBV菌株。S1基因分析表明，该病毒在进化过程中发生了重组事件。此外，实验感染表明，IBV分离株近年来在中国具有肾致病性。结论：S1蛋白基因的突变、插入、缺失和重组导致了中国IBV的遗传多样性。多种IBV在中国野外条件下的共同传播使其流行病学和演变非常复杂，表明开发新疫苗或疫苗策略的必要性。版权所有(C) 2009 S. Karger AG，巴塞尔
Objective: To monitor and study the molecular epidemiology, evolution and pathogenicity of infectious bronchitis viruses (IBVs) in China in recent years and further our knowledge of the evolution of IBVs. Methods: Thirty-seven IBV isolates were isolated from commercial chickens in China. The isolates were characterized by RT-PCR, sequencing, typing and analyzing the entire S1 gene. In addition, 4 selected IBV isolates were used to experimentally infect the specific pathogen-free chickens to study their pathogenicity. Results: Three types of IBV have been cocirculating in chicken flocks in China in recent years. Unique insertions and deletions in S1 protein regions were identified among different types of IBV. Moreover, a new IBV strain was isolated and identified in a layer hen. S1 gene analysis showed that a recombination event had occurred in the virus's evolutionary process. In addition, experimental infection has shown that IBV isolates have been nephropathogenic in China in recent years. Conclusion: Mutations, insertions, deletions and recombination of the S1 protein gene contribute to the genetic diversity of IBV in China. Cocirculation of multiple types of IBV in field conditions in China renders its epidemiology and evolution very complicated, indicating the necessity for development of new vaccines or vaccine strategies. Copyright (C) 2009 S. Karger AG, Basel