Rectal Administration of Lactobacillus casei DG Modifies Flora Composition and Toll-Like Receptor Expression in Colonic Mucosa of Patients with Mild Ulcerative Colitis
Rectal Administration of Lactobacillus casei DG Modifies Flora Composition and Toll-Like Receptor Expression in Colonic Mucosa of Patients with Mild Ulcerative Colitis
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DOI：
10.1007/s10620-010-1384-1
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发表时间：
2011-04-01
影响因子：
3.1
通讯作者：
Sturniolo, Giacomo Carlo
中科院分区：
文献类型：
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作者：
D'Inca, Renata;Barollo, Michela;Sturniolo, Giacomo Carlo
肠道微生物群的失衡似乎有助于胃肠道慢性炎症性疾病的发展，如溃疡性结肠炎（UC）。虽然已经提出益生菌补充剂是治疗结肠炎的有效方法，但其对肠道植物群和粘膜细胞因子平衡的影响从未被探索过。将26例轻度左侧UC患者随机分为3组，治疗8周：第一组7例患者单独口服5-氨基水杨酸（5-阿萨），第二组8例患者口服5-阿萨+L.第三组11例，口服5-阿萨，直肠注射L。卡塞湾从乙状结肠区采集活检标本，培养粘膜相关微生物，并通过定量实时聚合酶链反应（RT-PCR）评估细胞因子和TLR信使RNA（mRNA）水平。casei DG未能显著影响结肠植物群和TLR表达，但当与直肠给药L. casei DG，其通过增加乳杆菌属（Lactobacillus spp.）和减少肠杆菌。它还显著降低TLR-4和白细胞介素（IL）-1 β mRNA水平，并显著增加粘膜IL-10。干酪素DG及其对粘膜免疫系统的作用似乎是介导UC患者中益生菌的有益活性所必需的。
An imbalance in gut microbiota seems to contribute to the development of chronic inflammatory disorders of the gastrointestinal tract, such as ulcerative colitis (UC). Although it has been suggested that probiotic supplementation is an effective approach to colitis, its effects on intestinal flora and on mucosal cytokine balance have never been explored.To evaluate the effect of Lactobacillus casei (L. casei) DG, a probiotic strain, on colonic-associated microbiota, mucosal cytokine balance, and toll-like receptor (TLR) expression.Twenty-six patients with mild left-sided UC were randomly allocated to one of three groups for an 8-week treatment period: the first group of 7 patients received oral 5-aminosalicylic acid (5-ASA) alone, the second group of 8 patients received oral 5-ASA plus oral L. casei DG, and the third group of 11 patients received oral 5-ASA and rectal L. casei DG. Biopsies were collected from the sigmoid region to culture mucosal-associated microbes and to assess cytokine and TLR messenger RNA (mRNA) levels by quantitative real-time polymerase chain reaction (RT-PCR).5-ASA alone or together with oral L. casei DG failed to affect colonic flora and TLR expression in a significant manner, but when coupled with rectally administered L. casei DG, it modified colonic microbiota by increasing Lactobacillus spp. and reducing Enterobacteriaceae. It also significantly reduced TLR-4 and interleukin (IL)-1 beta mRNA levels and significantly increased mucosal IL-10.Manipulation of mucosal microbiota by L. casei DG and its effects on the mucosal immune system seem to be required to mediate the beneficial activities of probiotics in UC patients.