Estimation of the Potential of an Anaerobic Thermophilic Oil-Degrading Bacterium as a Candidate for MEOR
Estimation of the Potential of an Anaerobic Thermophilic Oil-Degrading Bacterium as a Candidate for MEOR
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厌氧高温石油降解细菌作为 MEOR 候选者的潜力估计
DOI：
10.1007/s13202-013-0095-5
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发表时间：
2014
影响因子：
2.2
通讯作者：
Kyuro Sasaki
中科院分区：
文献类型：
--
作者：
Isty A. Purwasena;Yuichi Sugai;Kyuro Sasaki
我们研究了原油的生物降解所造成的原油粘度的降低由细菌（AR 80）从油藏中分离，并估计这种细菌用于微生物提高石油采收率（MEOR）的潜力。AR 80优先降解长链正构烷烃，并在厌氧条件下增加原油中短链和长链正构烷烃浓度之比。AR 80的长链烷烃代谢作用降低了原油粘度。AR 80在补充有酵母提取物的盐水储层中生长良好，并且将油粘度降低至其原始值的约60%。添加少量酵母抽提物（0.05 g/L）对AR 80活性的刺激是必要的。因此，使用AR 80可以实现MEOR，而不会产生过高的成本。AR 80可以在高达80 °C的温度下生长，并在50至70 °C之间生长良好。AR 80可以在高达90 g/L的盐度下生长，在<30 g/L的盐度下生长良好。高压（如6.0 MPa）对AR 80活性影响不大。通过将AR 80（在补充有酵母提取物的盐水中）注入Berea砂岩岩心进行岩心驱替实验。流出油的气相色谱分析表明，长链正构烷烃的残余油中优先降解的AR 80在多孔岩石。岩心驱油实验表明，AR 80在多孔岩石中的活性提高了原油采收率，因此AR 80可以作为微生物采油的合适候选物。
We investigated the decrease in oil viscosity caused by the biodegradation of crude oil by a bacterium (AR80) isolated from an oil reservoir, and estimated the potential for this bacterium for use in microbial enhanced oil recovery (MEOR). AR80 degraded long-chainn-alkanes preferentially, and anaerobically increased the ratio between the short-chain and long-chainn-alkane concentrations in the crude oil. The long-chainn-alkane metabolism by AR80 decreased the oil viscosity. AR80 grew well in a reservoir of brine supplemented with yeast extract, and decreased the oil viscosity to approximately 60 % of its original value. Adding a small amount of yeast extract (0.05 g/L) was necessary to stimulate the AR80 activity. MEOR can, therefore, be achieved using AR80 without incurring excessive costs. AR80 can grow at temperatures up to 80 °C and grows well at between 50 and 70 °C. AR80 can grow at a salinity of up to 90 g/L and grows well at a salinity of <30 g/L. The AR80 activity was not affected very much by high pressures (such as 6.0 MPa). Core flooding experiments were performed by injecting AR80 (in brine supplemented with yeast extract) into Berea sandstone cores. Gas chromatography analysis of the effluent oil suggested that long-chainn-alkanes in the residual oil were preferentially degraded by the AR80 in the porous rocks. The core flooding experiments showed that the AR80 activity in the porous rocks caused the oil recovery to be enhanced, so AR80 could be a suitable candidate for MEOR.