A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species.
A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species.
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DOI：
10.1039/d3cc00462g
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发表时间：
2023-06
影响因子：
4.9
通讯作者：
Kai-Cheng Yan;J. Gardiner;A. Sedgwick;N. Thet;R. Heylen;T. James;A. Jenkins;Xiao‐Peng He
中科院分区：
文献类型：
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作者：
Kai-Cheng Yan;J. Gardiner;A. Sedgwick;N. Thet;R. Heylen;T. James;A. Jenkins;Xiao‐Peng He
使用具有长波长荧光发射的硝基还原酶 (NTR) 响应荧光探针来测定选定细菌物种在一系列不同细菌生长条件下的 NTR 活性，确保其在多种复杂临床环境下的适用性，其中灵敏度、反应时间和检测精度适合浮游培养物和生物膜。
A nitroreductase (NTR) responsive fluorescent probe with long wavelength fluorescence emission was used to determine the NTR activity of a selection of bacterial species under a range of different bacterial growth conditions ensuring applicability under multiple complex clinical environments, where sensitivity, reaction time, and the detection accuracy were suitable for planktonic cultures and biofilms.