THE HUMAN CYTOMEGALOVIRUS-86K IMMEDIATE-EARLY (IE) 2-PROTEIN REQUIRES THE BASIC REGION OF THE TATA-BOX BINDING-PROTEIN (TBP) FOR BINDING, AND INTERACTS WITH TBP AND TRANSCRIPTION FACTOR TFIIB VIA REGIONS OF IE2 REQUIRED FOR TRANSCRIPTIONAL REGULATION
THE HUMAN CYTOMEGALOVIRUS-86K IMMEDIATE-EARLY (IE) 2-PROTEIN REQUIRES THE BASIC REGION OF THE TATA-BOX BINDING-PROTEIN (TBP) FOR BINDING, AND INTERACTS WITH TBP AND TRANSCRIPTION FACTOR TFIIB VIA REGIONS OF IE2 REQUIRED FOR TRANSCRIPTIONAL REGULATION
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DOI：
10.1099/0022-1317-74-12-2691
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发表时间：
1993-12-01
影响因子：
3.8
通讯作者：
SINCLAIR, J
中科院分区：
文献类型：
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作者：
CASWELL, R;HAGEMEIER, C;SINCLAIR, J
人巨细胞病毒86K即刻早期(IE)2蛋白反式激活多个同源和异源启动子，包括70K热休克蛋白的细胞启动子(HSP70)和人类免疫缺陷病毒长末端重复序列。我们先前已经证明，IE2以TATA依赖的方式反式激活这两个启动子，并且在体外IE2能够与TATA盒结合蛋白(TBP)形成直接接触。我们现在证明IE2与TBP的基本重复区域结合。此外，IE2还可以接触第二个通用转录因子TFIIB。我们已经定位了IE2内的TBP和TFIIB结合区，并表明这些区域重叠，也位于先前确定的IE2反式激活和自动调节功能所需的蛋白质部分。
The 86K immediate early (IE) 2 protein of human cytomegalovirus trans-activates a number of homologous and heterologous promoters, including the cellular promoter for the 70K heat-shock protein (hsp70), and the human immunodeficiency virus long terminal repeat. We have previously shown that IE2 trans-activates these two promoters in a TATA-dependent manner, and that IE2 is able to form a direct contact with TATA-box binding protein (TBP) in vitro. We now show that IE2 binds to the basic repeat region of TBP. In addition IE2 can contact a second general transcription factor, TFIIB. We have mapped the TBP- and TFIIB-binding regions within IE2 and show that these regions overlap, and also lie within parts of the protein previously identified as being required for the trans-activation and autoregulation functions of IE2.