Late feeding in the active period decreases slow-wave activity.
Late feeding in the active period decreases slow-wave activity.
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DOI：
10.1016/j.lfs.2016.07.005
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发表时间：
2016-09
期刊：
影响因子：
6.1
通讯作者：
Kanna Oura;Airi Otsuka;T. Shiuchi;S. Chikahisa;Noriyuki Shimizu;H. Sei
中科院分区：
文献类型：
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作者：
Kanna Oura;Airi Otsuka;T. Shiuchi;S. Chikahisa;Noriyuki Shimizu;H. Sei
睡眠和进食行为密切相关，以维持能量平衡。虽然众所周知，睡眠障碍会导致各种代谢问题，如胰岛素抵抗，但对这种影响的机制知之甚少。因此，我们研究了活动期不同的进食节律是否影响睡眠-觉醒调节。主要方法2周内，小鼠被随机分配到3个喂养方案中的一个，如下：活动期自由进食实验室食物，（ZT 12 -24，Ad-lib组），在活动期的前半段自由进食实验室饲料（ZT 12 -18;早晨组），或在活动期的后半段自由进食实验室食物（ZT 18 -24，晚上组）。评估食物摄入、体重、体温、自发活动和睡眠。死后检查下丘脑和大脑皮层。关键发现三组之间的摄食量和体重没有观察到变化。晚上组慢波活动（SWA）低于其他2组，除了下丘脑中食欲素mRNA的表达较高，大脑皮层中多巴胺及其代谢产物的浓度较高。AMPK磷酸化增加，在下丘脑的小鼠在晚上组，然而，AMPK抑制没有影响SWA.SignificanceWe的结论是，晚喂养减少SWA在NREM睡眠通过一种机制，涉及食欲素介导的唤醒下丘脑和大脑皮层中的单胺升高。这些数据对睡眠-觉醒障碍和代谢紊乱之间的关系具有重要意义。
AimsSleep and feeding behaviors closely interact to maintain energy homeostasis. While it is known that sleep disorders can lead to various metabolic issues such as insulin resistance, the mechanism for this effect is poorly understood. We thus investigated whether different feeding rhythms during the active period affect sleep-wake regulation.Main methodsFor 2 weeks, mice were randomly assigned to 1 of 3 feeding schedules as follows: free access to lab chow during the active period (ZT12-24, Ad-lib group), free access to lab chow during the first half of the active period (ZT12-18; Morning group), or free access to lab chow during the second half of the active period (ZT18-24, Evening group). Food intake, body weight, body temperature, locomotor activity, and sleep were evaluated. The hypothalamus and cerebral cortex were examined post-mortem.Key findingsNo alterations in food intake or body weight were observed among the 3 groups. The Evening group showed lower slow-wave activity (SWA) than the other 2 groups, in addition to higher expression of orexin mRNA in the hypothalamus and higher concentrations of dopamine and its metabolites in the cerebral cortex. AMPK phosphorylation was increased in the hypothalamus of mice in the Evening group; however, AMPK inhibition had no effect on SWA.SignificanceWe concluded that late feeding reduces SWA in NREM sleep via a mechanism that involves orexin-mediated arousal in the hypothalamus and elevated monoamines in the cerebral cortex. These data have important implications for the relationship between sleep-wake disturbances and metabolic disorders.