Wound Healing and Angiogenesis through Combined Use of a Vascularized Tissue Flap and Adipose-Derived Stem Cells in a Rat Hindlimb Irradiated Ischemia Model
Wound Healing and Angiogenesis through Combined Use of a Vascularized Tissue Flap and Adipose-Derived Stem Cells in a Rat Hindlimb Irradiated Ischemia Model
复制标题
DOI：
10.1097/prs.0000000000002062
复制
发表时间：
2016-05-01
影响因子：
3.6
通讯作者：
Akita, Sadanori
中科院分区：
文献类型：
--
作者：
Yoshida, Shuhei;Yoshimoto, Hiroshi;Akita, Sadanori
背景资料：严重的肢体缺血的治疗有时是困难的，因为病人的病情，和一些新的approaches是needed.Methods：Sprague-Dawley大鼠的后肢，20-戈伊X射线照射和手术闭塞后，被分为四组：与浅筋膜瓣，5.0 × 10（6）脂肪来源的基质/干细胞，和两者相结合。检测大鼠的激光组织血流量、免疫组织学血管密度和足爪穿孔伤口愈合情况。绿色荧光蛋白标记的Sprague-Dawley大鼠被用于进一步的调查，通过细胞追踪2 weeks.Results：激光组织血流表现出显着增加，在联合治疗皮瓣和脂肪来源的干细胞在1和2周。单独用皮瓣治疗的治疗组和单独用脂肪源性干细胞治疗的治疗组之间没有显著差异。联合治疗1周后伤口愈合显著增加，除无皮瓣和无脂肪来源干细胞组外，2周时无伤口。在联合治疗组中，血管性血友病因子描述的血管数量在1周和2周时均显著增加。在细胞追踪组，在2周，绿色荧光蛋白标记的脂肪干细胞显着更积极的无皮瓣组比在flap group.Conclusions：脂肪来源的干细胞可能是一个强大的细胞来源，在照射和闭塞的肢体，通过提高组织血流量和血管密度。脂肪源性干细胞可能在一些困难的缺血性条件下在伤口愈合方面发挥重要作用。
Background: Treatment of critical limb ischemia is sometimes difficult because of the patient's condition, and some novel approaches are needed.Methods: The hindlimbs of Sprague-Dawley rats, after 20-Gy x-ray irradiation and surgical occlusion, were divided into four groups: with a superficial fascial flap, 5.0 x 10(6) adipose-derived stromal/stem cells, and both combined. The rats were tested for laser tissue blood flow, immunohistologic blood vessel density, and foot paw punch hole wound healing. Green fluorescent protein-tagged Sprague-Dawley rats were used for further investigation by cell tracking for 2 weeks.Results: Laser tissue blood flow demonstrated a significant increase in the combined treatment of flap and adipose-derived stem cells at both 1 and 2 weeks. There were no significant differences between the treatment groups treated with flaps alone and those treated with adipose-derived stem cells alone. Wound healing was significantly increased following combined treatment at 1 week, and there was no wound by 2 weeks except for the no-flap and no-adipose-derived stem cell group. The number of vessels depicted by von Willebrand factor showed a significant increase in the combined treatment group, at both 1 week and 2 weeks. In the cell tracking group, at 2 weeks, the green fluorescent protein-tagged adipose-derived stem cells were significantly more positive in the no-flap group than in the flap group.Conclusions: Adipose-derived stem cells may be a potent cell source in irradiated and occluded limbs by enhancing tissue blood flow and blood vessel density. Adipose-derived stem cells may play an important role in some difficult ischemic conditions in terms of wound healing.