Immunophenotypic analysis of hematogones (B-lymphocyte precursors) in 662 consecutive bone marrow specimens by 4-color flow cytometry
Immunophenotypic analysis of hematogones (B-lymphocyte precursors) in 662 consecutive bone marrow specimens by 4-color flow cytometry
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DOI：
10.1182/blood.v98.8.2498
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发表时间：
2001-10-15
期刊：
影响因子：
20.3
通讯作者：
Kroft, SH
中科院分区：
文献类型：
--
作者：
McKenna, RW;Washington, LT;Kroft, SH
骨髓造血素(B淋巴细胞前体)可能会引起诊断问题，因为它们在形态和免疫表型上与肿瘤性淋巴母细胞相似。这项前瞻性的多参数流式细胞术研究的目的是量化不同年龄组和不同临床状况的血球，确定影响血球相对数量的因素，并将其免疫表型与肿瘤淋巴母细胞进行比较。使用两种四色抗体组合中的一种对662份连续的骨髓样本进行了血原性分析，其中528份(79.8%)被鉴定出血原性。随着年龄的增长，血糖值显著下降(P<；.001)，但在所有年龄段都有很大的变化范围，许多成年人的血糖值相对较高。密度梯度处理的样本比红细胞裂解处理的样本有更高的平均血原率(P<；.001)。随着骨髓受累肿瘤细胞的增加，血球直接减少。在662份样本中，总共有8%的样本含有5%或以上的血原：24.6%的样本来自16岁以下的患者，6.3%的样本来自16岁及以上的患者(P<；.000 01)。在淋巴瘤、骨髓再生状态、免疫性细胞减少症和获得性免疫缺陷综合征的患者中，最常观察到的是红细胞生成量增加。造血细胞总是表现出典型的复杂的抗原表达谱，定义了B细胞前体的正常抗原进化，缺乏异常表达。相比之下，49例B-ALL前体淋巴细胞成熟停滞，免疫表型异常1~11例。具有最佳抗体组合的四色流式细胞术一致地区分血球和肿瘤性淋巴母细胞。(血。2001年；98：2498-2507)(C)2001，由美国血液病学会主办。
Bone marrow hematogones (B-lymphocyte precursors) may cause problems in diagnosis because of their morphologic and immunophenotypic similarities to neoplastic lymphoblasts. The purposes of this prospective, multiparametric flow cytometry study were to quantify hematogones across age groups and a spectrum of clinical conditions, to identify factors that affect the relative quantity of hematogones, and to compare their immunophenotype with that of neoplastic lymphoblasts. A total of 662 consecutive marrow specimens were analyzed for hematogones using one of two 4-color antibody combinations; hematogones were identified in 528 (79.8%). There was a significant decline in hematogones with increasing age (P < .001), but a broad range was found at all ages and many adults had a relatively high number. Specimens processed by density gradient had a higher mean percent hematogones than those processed by erythrocyte lysis (P < .001). There was a direct decline in hematogones with increasing marrow involvement with neoplastic cells. A total of 8% of the 662 specimens contained 5% or more hematogones: 24.6% of specimens from patients aged less than 16 years and 6.3% from those 16 and older (P < .000 01). Increased hematogones were observed most often in patients with lymphoma, marrow regenerative states, immune cytopenias, and acquired immunodeficiency syndrome. Hematogones always exhibited a typical complex spectrum of antigen expression that defines the normal antigenic evolution of B-cell precursors and lacked aberrant expression. In contrast, lymphoblasts in 49 cases of precursor B-ALL showed maturation arrest and exhibited 1 to 11 immunophenotypic aberrancies. Four-color flow cytometry with optimal combinations of antibodies consistently distinguishes between hematogones and neoplastic lymphoblasts. (Blood. 2001;98:2498-2507) (C) 2001 by The American Society of Hematology.