STRETCHING DNA WITH A RECEDING MENISCUS - EXPERIMENTS AND MODELS
STRETCHING DNA WITH A RECEDING MENISCUS - EXPERIMENTS AND MODELS
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DOI：
10.1103/physrevlett.74.4754
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发表时间：
1995-06-05
影响因子：
8.6
通讯作者：
BENSIMON, A
中科院分区：
文献类型：
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作者：
BENSIMON, D;SIMON, AJ;BENSIMON, A
提出了通过移动半月板对移植 DNA 分子进行排列的详细实验和理论分析。拉伸的存在和程度（高达未拉伸长度的 2.14 倍）主要取决于表面的特性。两端接枝的分子呈现出尺度不变的环状形状。引入了该过程的弹性模型（我们称之为分子梳理），该模型 (a) 产生不同表面上的延伸力，(b) 产生 DNA 拉伸强度值，476±84 pN，(c) 描述没有拟合参数的环的形状。
A detailed experimental and theoretical analysis of the alignment of grafted DNA molecules by a moving meniscus is presented. The existence and extent of the stretching (up to 2.14 times the unstretched length) depends critically on the properties of the surface. Molecules grafted at both ends exhibit a looplike shape which is scale invariant. An elastic model of this process, which we have called molecular combing, is introduced which (a) yields the extension force on various surfaces,(b) yields a value for the tensile strength of DNA, 476±84 pN, and (c) describes the shape of the loops with no fitting parameters.