FURTHER VALIDATION OF FETAX - EVALUATION OF THE DEVELOPMENTAL TOXICITY OF 5 KNOWN MAMMALIAN TERATOGENS AND NON-TERATOGENS
FURTHER VALIDATION OF FETAX - EVALUATION OF THE DEVELOPMENTAL TOXICITY OF 5 KNOWN MAMMALIAN TERATOGENS AND NON-TERATOGENS
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DOI：
10.3109/01480549009032286
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发表时间：
1990-01-01
影响因子：
2.6
通讯作者：
BUSH, SJ
中科院分区：
文献类型：
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作者：
BANTLE, JA;FORT, DJ;BUSH, SJ
使用青蛙胚胎致畸试验：非洲爪蟾 (FETAX) 评估五种化合物的发育毒性。晚期非洲爪蟾囊胚暴露于 5-氮杂胞苷、甲氨蝶呤、伪麻黄碱、阿斯巴甜和苋菜中 96 小时。对每种化合物进行了三个独立的静态更新测定。根据致畸指数[LC50/EC50（畸形）]值、诱发畸形的类型和严重程度以及胚胎生长，5-氮杂胞苷和甲氨蝶呤经测试具有很强的致畸潜力。伪麻黄碱具有中等致畸潜力，但苋菜和阿斯巴甜几乎没有致畸潜力。结果支持使用 FETAX 筛选发育毒物。
The developmental toxicity of five compounds was evaluated with the Frog Embryo Teratogenesis Assay: Xenopus (FETAX). Late Xenopus laevis blastulae were exposed to 5-azacytidine, methotrexate, pseudoephedrine, aspartame, and amaranth for 96 h. Three separate static-renewal assays were conducted for each compound. Based on Teratogenic Index [LC50/EC50 (malformation)] values, types and severity of induced malformations, and embryo growth, 5-azacytidine and methotrexate tested as having strong teratogenic potential. Pseudoephedrine scored as having moderate teratogenic potential, but amaranth and aspartame had little or no teratogenic potential. Results support the use of FETAX for the screening of devleopmental toxicants.