Multiple interactions amongst floral homeotic MADS box proteins
Multiple interactions amongst floral homeotic MADS box proteins
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DOI：
10.1002/j.1460-2075.1996.tb00807.x
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发表时间：
1996-08-15
期刊：
影响因子：
11.4
通讯作者：
Sommer, H
中科院分区：
文献类型：
--
作者：
Davies, B;EgeaCortines, M;Sommer, H
大多数已知的花器官同源异型基因属于MADS盒家族，它们的产物通过一种未知的机制联合作用来指定花器官的特性。我们使用酵母双杂交系统研究了金鱼草器官识别基因产物之间的相互作用网络，观察到MADS盒因子之间的选择性异二聚化，检测到两个因子DEFICIENS(DEF)和GLO(GLO)之间的排他性相互作用，这两个因子以前被认为是异二聚体和控制花瓣和雄蕊的发育，相比之下，第三个肥胖者Plena(PLE)是生殖器官发育所必需的，它可以与MADS盒基因表达的产物在早、中、晚阶段相互作用。我们还证明了DEF和GLO的异二聚化需要K盒，这是非植物MADS盒因子中没有的区域，这表明植物MADS盒因子可能有不同的相互作用标准，Plena和时间中间的MADS盒因子的关联表明它们在分生组织和器官识别基因之间的部分调节功能是通过直接相互作用完成的，这些数据揭示了控制花发育的因子之间出人意料的复杂的相互作用网络，这对器官特性的确定具有重要意义。
Most known floral homeotic genes belong to the MADS box family and their products act in combination to specify floral organ identity by an unknown mechanism. We have used a yeast two-hybrid system to investigate the network of interactions between the Antirrhinum organ identity gene products, Selective heterodimerization is observed between MADS box factors, Exclusive interactions are detected between two factors, DEFICIENS (DEF) and GLOBOSA (GLO), previously known to heterodimerize and control development of petals and stamens, In contrast, a third fatter, PLENA (PLE), which is required for reproductive organ development, can interact with the products of MADS box genes ex-pressed at early, intermediate and late stages. We also demonstrate that heterodimerization of DEF and GLO requires the K box, a domain not found in non-plant MADS box factors, indicating that the plant MADS box factors mag have different criteria for interaction, The association of PLENA and the temporally intermediate MADS box factors suggests that part of their function in mediating between the meristem and organ identity genes is accomplished through direct interaction, These data reveal an unexpectedly complex network of interactions between the factors controlling flower development and have implications for the determination of organ identity.