IgE+ memory B cells and plasma cells generated through a germinal-center pathway
IgE+ memory B cells and plasma cells generated through a germinal-center pathway
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DOI：
10.1038/ni.2256
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发表时间：
2012-04-01
影响因子：
30.5
通讯作者：
Wu, Lawren C.
中科院分区：
文献类型：
--
作者：
Talay, Oezcan;Yan, Donghong;Wu, Lawren C.
免疫球蛋白E（IgE）抗体在哮喘和过敏性疾病中是致病性的，但对IgE产生（IgE（+））细胞的体内生物学知之甚少。IgE（+）B细胞分化的模型提出，IgE（+）细胞通过生殖中心IgG 1（+）中间体发育，IgE记忆存在于IgG 1（+）记忆B细胞的隔室中。在这里，我们已经使用了一个报告小鼠表达绿色荧光蛋白与膜IgE转录（IgE-GFP），以评估在体内IgE反应。与以IgG 1为中心的IgE转换和记忆模型相反，我们发现IgE+细胞通过生殖中心的IgE（+）中间体发育形成IgE（+）记忆B细胞和浆细胞。我们的研究为IgE转换和记忆的体内生物学描绘了一个新的模型。
Immunoglobulin E (IgE) antibodies are pathogenic in asthma and allergic diseases, but the in vivo biology of IgE-producing (IgE(+)) cells is poorly understood. A model of the differentiation of IgE(+) B cells proposes that IgE(+) cells develop through a germinal-center IgG1(+) intermediate and that IgE memory resides in the compartment of IgG1(+) memory B cells. Here we have used a reporter mouse expressing green fluorescent protein associated with membrane IgE transcripts (IgE-GFP) to assess in vivo IgE responses. In contrast to the IgG1-centered model of IgE switching and memory, we found that IgE+ cells developed through a germinal-center IgE(+) intermediate to form IgE(+) memory B cells and plasma cells. Our studies delineate a new model for the in vivo biology of IgE switching and memory.