Laser Machined Fiber-Based Microprobe: Application in Microscale Electroporation
Laser Machined Fiber-Based Microprobe: Application in Microscale Electroporation
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DOI：
10.1007/s42765-022-00148-5
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发表时间：
2022-03-23
影响因子：
16.1
通讯作者：
Jia, Xiaoting
中科院分区：
文献类型：
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作者：
Kim, Jongwoon;Zhao, Yajun;Jia, Xiaoting
微型电穿孔装置大多限于体外实验（即，微通道和微毛细管）。新型的基于纤维的微探针能够在体内进行微尺度电穿孔，并任意选择感兴趣的细胞群进行电穿孔。我们开发了一种灵活的，基于纤维的微尺度电穿孔装置，通过热拉伸过程和飞秒激光微加工技术。该光纤由四个铜电极（80 μ m）、一个微流体通道（30 μ m）组成，并且具有400 μ m的总直径。暴露的电极和通道的尺寸可通过精密的飞秒激光设置进行定制。通过数值模拟和体外实验验证了光纤探针的可行性。使用荧光染色在3D胶原支架（接种有U251人胶质瘤细胞）中观察到成功的可逆和不可逆的微尺度电穿孔。采用协方差误差椭圆法对消融区域进行了估计，并与数值模拟结果进行了比较。基于光纤的工作微探针的计算和实验结果表明，在脑深部等空间敏感区域进行体内微尺度电穿孔是可行的。
Microscale electroporation devices are mostly restricted to in vitro experiments (i.e., microchannel and microcapillary). Novel fiber-based microprobes enable in vivo microscale electroporation and arbitrarily select the cell groups of interest to electroporate. We developed a flexible, fiber-based microscale electroporation device through a thermal drawing process and femtosecond laser micromachining techniques. The fiber consists of four copper electrodes (80 mu m), one microfluidic channel (30 mu m), and has an overall diameter of 400 mu m. The dimensions of the exposed electrodes and channel were customizable through a delicate femtosecond laser setup. The feasibility of the fiber probe was validated through numerical simulations and in vitro experiments. Successful reversible and irreversible microscale electroporation was observed in a 3D collagen scaffold (seeded with U251 human glioma cells) using fluorescent staining. The ablation regions were estimated by performing the covariance error ellipse method and compared with the numerical simulations. The computational and experimental results of the working fiber-based microprobe suggest the feasibility of in vivo microscale electroporation in space-sensitive areas, such as the deep brain.