Aptamer-Functionalized Magnetic Conjugated Organic Framework for Selective Extraction of Traces of Hydroxylated Polychlorinated Biphenyls in Human Serum
Aptamer-Functionalized Magnetic Conjugated Organic Framework for Selective Extraction of Traces of Hydroxylated Polychlorinated Biphenyls in Human Serum
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DOI：
10.1002/chem.201800092
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发表时间：
2018-07-20
影响因子：
4.3
通讯作者：
Jia, Qiong
中科院分区：
文献类型：
--
作者：
Jiang, Dandan;Hu, Tingting;Jia, Qiong
研制了一种基于适配体功能化磁共轭有机骨架（COF）的固相萃取吸附剂，用于选择性萃取痕量羟基化多氯联苯。该材料具有磁芯的超顺磁性、COF的高比表面积和多孔结构以及适配体的高比亲和力等优点。结合高效液相色谱-质谱，采用适体功能化磁性COF法捕获人血清中羟基-2,3,4,5,5-五氯联苯。方法在0.01 ~ 40 ngmL（-1）的线性范围内具有良好的相关系数（R-2=0.9973）。检出限低至2.1 pgmL（-1）。此外，该材料具有良好的可重复使用性，可用于至少10次提取循环，回收率大于90%。
A solid-phase extraction adsorbent based on an aptamer-functionalized magnetic conjugated organic framework (COF) was developed for selective extraction of traces of hydroxylated polychlorinated biphenyls. This material has advantages such as superparamagnetism of the magnetic core, high surface area and porous structure of the COF, and high specific affinity of the aptamer. In combination with HPLC-MS, the aptamer-functionalized magnetic COF was used for the capture of hydroxy-2,3,4,5,5-pentachlorobiphenyl in human serum. The method provided a linear range of 0.01-40 ngmL(-1) with a good correlation coefficient (R-2=0.9973). The limit of detection was as low as 2.1 pgmL(-1). Furthermore, the material showed good reusability and could be applied in at least ten extraction cycles with recoveries greater than 90%.