Photoaptamer arrays applied to multiplexed proteomic analysis
Photoaptamer arrays applied to multiplexed proteomic analysis
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DOI：
10.1002/pmic.200300631
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发表时间：
2004-03-01
期刊：
影响因子：
3.4
通讯作者：
Zichi, D
中科院分区：
文献类型：
--
作者：
Bock, C;Coleman, M;Zichi, D
描述了基于多重光适体的阵列，该阵列允许同时测量血清样品中多种感兴趣的蛋白质。由于光适体在荧光信号检测之前与其目标分析物共价结合，因此可以大力清洗阵列以去除背景蛋白质，从而提供了在生物基质中获得优异的信噪比和更低的定量限的潜力。这里提供了 17 重光适体阵列的数据，其对多种分析物（包括白细胞介素 16、血管内皮生长因子和内皮抑素）的检测限低于 10 fM，并且能够测量 10% 血清样品中的蛋白质。该检测方法简单、可扩展且可重复。捕获试剂的亲和力与阵列上分析物的检测限直接相关。
Multiplexed photoaptamer-based arrays that allow for the simultaneous measurement of multiple proteins of interest in serum samples are described. Since photoaptamers covalently bind to their target analytes before fluorescent signal detection, the arrays can be vigorously washed to remove background proteins, providing the potential for superior signal-to-noise ratios and lower limits of quantification in biological matrices. Data are presented here for a 17-plex photoaptamer array exhibiting limits of detection below 10 fM for several analytes including interleukin-16, vascular endothelial growth factor, and endostatin and able to measure proteins in 10% serum samples. The assays are simple, scalable, and reproducible. Affinity of the capture reagent is shown to be directly correlated to the limit of detection for the analyte on the array.