Lectin histochemical HPA-binding pattern of human breast and colon cancers is associated with metastases formation in severe combined immunodeficient mice
Lectin histochemical HPA-binding pattern of human breast and colon cancers is associated with metastases formation in severe combined immunodeficient mice
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DOI：
10.1023/a:1026404832394
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发表时间：
1997-09-01
期刊：
影响因子：
--
通讯作者：
Adam, E
中科院分区：
文献类型：
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作者：
Schumacher, U;Adam, E
转移形成是癌症治疗中的主要临床问题，并且在转移扩散的治疗方面没有取得显著进展。这种明显缺乏进展的部分原因是缺乏临床相关的转移动物模型。凝集素Happenatia凝集素（HPA）的结合与乳腺癌和结肠癌患者的预后不良有关。将HPA阳性和阴性人乳腺癌和结肠癌细胞系移植到严重联合免疫缺陷（SCID）小鼠中。HPA阳性乳腺癌细胞系（MCF-7和T47 D）在SCID小鼠中转移，而HPA阴性乳腺癌细胞系（BT 20，HS 578 T和HBL 100）则没有。HPA阳性结肠癌细胞系HT 29发生转移，而HPA阴性结肠癌细胞系（COLO 320 DM、SW 480和SW 620）未发生转移。然而，在8只接种HPA阴性结肠癌细胞系的SCID小鼠中，有2只发现了CACO 2转移性沉积物，尽管有这个例外，HPA结合是SCID小鼠中人类乳腺癌和结肠癌细胞转移的良好指标：26只HPA阳性癌症中有23只发生了转移，而38只HPA阴性癌症中只有2只发生了转移。该实验模型非常适合于研究HPA识别的碳水化合物残基在乳腺癌和结肠癌转移中的功能作用。
Metastasis formation is a major clinical problem in cancer treatment, and no significant progress in the treatment of metastatic spread has been made. This apparent lack of progress is partly caused by the absence of clinically relevant animal models of metastases. The binding of the lectin Helix pomatia agglutinin (HPA) has been associated with a poor prognosis in breast and colon cancer patients. HPA-positive and -negative human breast and colon cancer cell lines were transplanted into severe combined immunodeficient (SCID) mice. HPA-positive breast cancer cell lines (MCF-7 and T47D) metastasized in SCID mice, whereas the HPA-negative ones (BT20, HS578T and HBL100) did not. The HPA-positive colon cancer cell line HT29 metastasized, while the HPA-negative ones (COLO320DM, SW480 and SW620) did not. However, in two of eight SCID mice inoculated with the HPA-negative colon cancer cell line, CACO2 metastatic deposits were found. Despite this exception, HPA binding is a good indicator of the metastasis of human breast and colon cancer cells in SCID mice: 23 out of 26 HPA-positive cancers metastasized, as opposed to only two out of 38 HPA-negative cancers. This experimental model is well suited for investigating the functional role of carbohydrate residues recognized by HPA in breast and colon cancer metastasis.