Nitrogen-fixing populations of Planctomycetes and Proteobacteria are abundant in surface ocean metagenomes.
Nitrogen-fixing populations of Planctomycetes and Proteobacteria are abundant in surface ocean metagenomes.
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DOI：
10.1038/s41564-018-0176-9
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发表时间：
2018-07
影响因子：
28.3
通讯作者：
Eren AM
中科院分区：
文献类型：
--
作者：
Delmont TO;Quince C;Shaiber A;Esen ÖC;Lee ST;Rappé MS;McLellan SL;Lücker S;Eren AM
表层海洋的固氮影响全球海洋氮的生物有效性，从而影响微生物的初级生产力。到目前为止，蓝藻种群一直被视为该栖息地生物有效氮的主要供应者。虽然针对固氮酶还原酶基因的PCR扩增调查揭示了各种非蓝藻重氮营养种群的存在，但随后的定量PCR调查表明，它们通常以低丰度出现。在这里，我们使用最先进的元基因组组装和入库策略，从Tara Ocean的元基因组中恢复近1000个非冗余的微生物种群基因组。其中，我们首次提供了居住在公海表层水域的非蓝藻重氮细菌的基因组证据，这些证据与变形杆菌和最引人注目的植物真菌的谱系相对应。后一门的成员普遍存在于水生系统中，但以前从未与固氮联系在一起。此外，利用全基因组定量阅读招募，我们证明了所发现的重氮菌不仅分布广泛，而且在太平洋和大西洋西北部都非常丰富(单个种群高达总基因组阅读的0.3%)。我们的结果延续了数十年基于聚合酶链式反应的基因调查，并证实了异养细菌在固定表层海洋中氮的重要性。从表层海洋样本中恢复的种群基因组发现了广泛和丰富的非蓝藻重氮营养物质，包括变形杆菌和植物真菌，表明它们对该环境中的固氮具有重要意义。
Nitrogen fixation in the surface ocean impacts global marine nitrogen bioavailability and thus microbial primary productivity. Until now, cyanobacterial populations have been viewed as the main suppliers of bioavailable nitrogen in this habitat. Although PCR amplicon surveys targeting the nitrogenase reductase gene have revealed the existence of diverse non-cyanobacterial diazotrophic populations, subsequent quantitative PCR surveys suggest that they generally occur in low abundance. Here, we use state-of-the-art metagenomic assembly and binning strategies to recover nearly one thousand non-redundant microbial population genomes from the TARA Oceans metagenomes. Among these, we provide the first genomic evidence for non-cyanobacterial diazotrophs inhabiting surface waters of the open ocean, which correspond to lineages within the Proteobacteria and, most strikingly, the Planctomycetes. Members of the latter phylum are prevalent in aquatic systems, but have never been linked to nitrogen fixation previously. Moreover, using genome-wide quantitative read recruitment, we demonstrate that the discovered diazotrophs were not only widespread but also remarkably abundant (up to 0.3% of metagenomic reads for a single population) in both the Pacific Ocean and the Atlantic Ocean northwest. Our results extend decades of PCR-based gene surveys, and substantiate the importance of heterotrophic bacteria in the fixation of nitrogen in the surface ocean. Recovery of population genomes from surface ocean samples identified non-cyanobacterial diazotrophs that were widespread and abundant, including Proteobacteria and Planctomycetes, indicating their importance for nitrogen fixation in this environment.
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影响因子：
5.2
作者：
Delmont TO;Eren AM;Maccario L;Prestat E;Esen ÖC;Pelletier E;Le Paslier D;Simonet P;Vogel TM
通讯作者：
Vogel TM
影响因子：
4.5
作者：
CARPENTER, EJ;HARBISON, GR;MCALISTER, VL
通讯作者：
MCALISTER, VL
影响因子：
56.9
作者：
CARPENTER, EJ;ROMANS, K
通讯作者：
ROMANS, K
影响因子：
2.7
作者：
Darling AE;Jospin G;Lowe E;Matsen FA 4th;Bik HM;Eisen JA
通讯作者：
Eisen JA
影响因子：
3.7
作者：
Creevey CJ;Doerks T;Fitzpatrick DA;Raes J;Bork P
通讯作者：
Bork P