Isolation, identification and characterization of a novel Rhodococcus sp. strain in biodegradation of tetrahydrofuran and its medium optimization using sequential statistics-based experimental designs.
Isolation, identification and characterization of a novel Rhodococcus sp. strain in biodegradation of tetrahydrofuran and its medium optimization using sequential statistics-based experimental designs.
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DOI：
10.1016/j.biortech.2009.01.006
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发表时间：
2009-06
影响因子：
11.4
通讯作者：
Yanlai Yao;Z. Lv;H. Min;Zhenhua Lv;Huipeng Jiao
中科院分区：
文献类型：
--
作者：
Yanlai Yao;Z. Lv;H. Min;Zhenhua Lv;Huipeng Jiao
基于统计学的实验设计应用于优化的培养条件，四氢呋喃（THF）降解的新分离的红球菌属。YYL，耐受高浓度的THF。通过单因素试验确定了K2 HPO 4. 3 H2O、NH 4Cl和酵母膏浓度、初始pH值等4个因素的最佳范围，并采用响应面法对这些因素进行了优化。Plackett-Burman设计用于鉴定显著增加THF降解速率的三种微量元素（Mg 2+、Zn 2+和Fe 2+）。最佳工艺条件为：NH 4Cl 1.80g/L，K2 HPO 4·3 H2O 0.81g/L，酵母膏0.06g/L，MgSO 4·7 H2O 0.40g/L，ZnSO 4·7 H2O 0.006g/L，FeSO 4·7 H2O 0.024g/L，初始pH 8.26。在此优化条件下，红球菌YYL的最大THF降解速率增加到137.60mg THFh-1g干重，这是先前描述的THF降解红球菌菌株的近5倍。
Statistics-based experimental designs were applied to optimize the culture conditions for tetrahydrofuran (THF) degradation by a newly isolated Rhodococcus sp. YYL that tolerates high THF concentrations. Single factor experiments were undertaken for determining the optimum range of each of four factors (initial pH and concentrations of K2HPO4·3H2O, NH4Cl and yeast extract) and these factors were subsequently optimized using the response surface methodology. The Plackett–Burman design was used to identify three trace elements (Mg2+, Zn2+and Fe2+) that significantly increased the THF degradation rate. The optimum conditions were found to be: 1.80g/L NH4Cl, 0.81g/L K2HPO4·3H2O, 0.06g/L yeast extract, 0.40g/L MgSO4·7H2O, 0.006g/L ZnSO4·7H2O, 0.024g/L FeSO4·7H2O, and an initial pH of 8.26. Under these optimized conditions, the maximum THF degradation rate increased to 137.60mg THFh−1g dry weight in Rhodococcus sp. YYL, which was nearly five times of that by the previously described THF degrading Rhodococcus strain.