Human epithelial cancers secrete immunoglobulin g with unidentified specificity to promote growth and survival of tumor cells.
Human epithelial cancers secrete immunoglobulin g with unidentified specificity to promote growth and survival of tumor cells.
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发表时间：
2003-10
期刊：
影响因子：
11.2
通讯作者：
Xiaoyan Qiu;Xiao-hui Zhu;Liang Zhang;Y. Mao;Jian Zhang;Peng Hao;Guohui Li;Peng Lv;Zhixin Li
中科院分区：
文献类型：
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作者：
Xiaoyan Qiu;Xiao-hui Zhu;Liang Zhang;Y. Mao;Jian Zhang;Peng Hao;Guohui Li;Peng Lv;Zhixin Li
免疫球蛋白（Ig）迄今为止仅被发现由分化的B淋巴细胞产生。通过免疫组化分析，原位杂交，和激光捕获显微切割辅助单细胞PCR，我们证明，人类上皮来源的癌症，包括乳腺癌，结肠癌，肝癌，肺癌，建立上皮癌细胞系，以及一些正常的肺组织，也产生IgG在细胞质和分泌形式。此外，通过反义DNA或抗人IgG抗体阻断肿瘤来源的IgG增加了程序性细胞死亡并抑制了体外癌细胞的生长。更重要的是，抗人IgG抗体的管理也抑制IgG分泌癌细胞系在免疫缺陷裸鼠的生长。我们的研究结果支持肿瘤源性IgG作为上皮癌生长因子的作用。IgG在人类肿瘤中的普遍表达及其促生长功能可能对人类肿瘤的生长调节和靶向治疗具有重要意义。
Immunoglobulins (Igs) are found thus far only to be produced by differentiated B lymphocytes. By immunohistochemistry analysis, in situ hybridization, and laser capture microdissection-assisted single-cell PCR, we demonstrate that human cancers of epithelial origin, including carcinomas of breast, colon, liver, lung, established epithelial cancer lines, as well as some normal lung tissues, also produce IgG in both cytoplasmic and secreted forms. Furthermore, blockade of tumor-derived IgG by either antisense DNA or antihuman IgG antibody increased programmed cell death and inhibited growth of cancer cells in vitro. More importantly, administration of antihuman IgG antibody also suppressed the growth of an IgG-secreting carcinoma line in immunodeficient nude mice. Our results support a role of tumor-derived IgG as growth factor for epithelial cancers. Prevalent expression of IgG in human carcinomas and its growth-promoting functions may have important implications in growth regulation and targeted therapy of human cancers.