Manipulations in hydrogel degradation behavior enhance osteoblast function and mineralized tissue formation
Manipulations in hydrogel degradation behavior enhance osteoblast function and mineralized tissue formation
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DOI：
10.1089/ten.2006.12.1663
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发表时间：
2006-06-01
影响因子：
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通讯作者：
Anseth, Kristi S.
中科院分区：
文献类型：
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作者：
Benoit, Danielle S. W.;Durney, Andrew R.;Anseth, Kristi S.
用聚乙二醇二甲基丙烯酸酯(PEGDM)与可降解大分子单体聚乳酸(PEGDM)共聚，制备了聚乳酸-b-聚乙二醇-b-甲基丙烯酸酯封端大分子单体(PEGDM-LA-DM)。共聚物网络由100：0、83：17、67：33和50：50PEGDM：PEG-LA-DM质量%组成，基本上形成了在实验过程中分别表现出0、17、33和50%降解的支架。将成骨细胞光包裹在这些共聚水凝胶中，体外培养3周。随着聚乙二醇LA-DM含量的增加和相应降解量的增加，代谢活性、增殖和碱性磷酸酶产量增加。对培养的成骨细胞进行β-肌动蛋白基因表达分析，骨桥蛋白和I型胶原基因的表达随着降解程度的增加而增加。最后，作为矿化组织形成的指标，对钙和磷的沉积进行了生化和组织学分析。矿化程度随着聚乙二醇LA-DM浓度的增加而增加，其生化性质与羟基磷灰石相似。
Hydrogels were prepared by copolymerizing a degradable macromer, poly(lactic acid)-b-poly(ethylene glycol)-b-poly(lactic acid) endcapped with methacrylate groups (PEG-LA-DM), with a nondegradable macromer, poly( ethylene glycol) dimethacrylate (PEGDM). Copolymer networks consisted of 100: 0, 83: 17, 67: 33, and 50: 50 PEGDM: PEG-LA-DM mass%, essentially creating scaffolds that exhibit 0, 17, 33, and 50% degradation over the time course of the experiment. Osteoblasts were photoencapsulated in these copolymer hydrogels and cultured for 3 weeks in vitro. Metabolic activity, proliferation, and alkaline phosphatase production were enhanced by an increase PEG-LA-DM content and corresponding degradation. Gene expression of the cultured osteoblasts, normalized to beta-actin, was analyzed, and osteopontin and collagen type I gene expression increased with degradation. Finally, as a measure of mineralized tissue formation, calcium and phosphate deposition was analyzed biochemically and histologically. Mineralization increased with increasing concentration of PEG-LA-DM and biochemically resembled that of hydroxyapatite.