Transcriptional analysis of Kluyveromyces marxianus for ethanol production from inulin using consolidated bioprocessing technology.
Transcriptional analysis of Kluyveromyces marxianus for ethanol production from inulin using consolidated bioprocessing technology.
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DOI：
10.1186/s13068-015-0295-y
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发表时间：
2015
影响因子：
6.3
通讯作者：
Bai F
中科院分区：
文献类型：
--
作者：
Gao J;Yuan W;Li Y;Xiang R;Hou S;Zhong S;Bai F
用非作物材料，如耶路撒冷生产乙醇，将对能源工业作出巨大贡献。非常规酵母，马克思克鲁维酵母，能够进行乙醇发酵的糖分子获得的含菊粉的材料，通过巩固的生物加工。较低的菊粉浓度和微通气可以导致相对快速和理想的发酵过程;然而，尚不清楚是什么原因导致较高浓度的菊粉的抑制和通气的促进作用。利用新一代测序技术研究了K. marxianus Y179在120 g/L菊粉不通气（120-N）、230 g/L菊粉不通气（230-N）、230 g/L菊粉通气（230- 130 mV）三种发酵条件下，通过控制ORP为-130 mV（230- 130 mV），对该菌株进行了初步发酵。 从三个样品中生成了总共3555万个干净的读段，其中4,820个预测开放阅读框被注释。在差异表达分析中，分别在230- 130 mV和120-N条件下发现950和1，452个差异表达基因，并将样品230-N用作对照。这些基因主要与中心碳代谢和乙醇形成途径相关。菊粉酶的表达增加和自噬相关基因ATG 8的低活性确保了快速和理想的发酵过程。尽管被报道为“crabtree阴性”物种，K。marxianus Y179在微通气和高菊糖浓度下具有良好的乙醇发酵特性。K. marxianus Y179细胞对菊糖浓度和微通气的反应参与了整个乙醇代谢网络。研究结果为进一步探索菊糖乙醇发酵的调控机制奠定了重要基础，也为进一步研究菊糖代谢网络的优化和重构提供了参考。马克思主义。本文的在线版本（doi：10.1186/s13068-015-0295-y）包含补充材料，可供授权用户使用。
Ethanol production from non-crop materials, such as Jerusalem artichokes, would make a great contribution to the energy industry. The non-conventional yeast, Kluyveromyces marxianus, is able to carry out ethanol fermentation of sugar molecules obtained from inulin-containing materials by consolidated bioprocessing. Lower inulin concentrations and micro-aeration can lead to a relatively fast and ideal fermentation process; however, it is unclear what causes the inhibition of higher concentrations of inulin and the promotion effect of aeration. Next-generation sequencing technology was used to study the global transcriptional response of K. marxianus Y179 under three fermentation conditions, including 120 g/L inulin without aeration (120-N), 230 g/L inulin without aeration (230-N), 230 g/L inulin with aeration by ORP controlling at −130 mV (230-130mV). A total of 35.55 million clean reads were generated from three samples, of which 4,820 predicted that open reading frames were annotated. For differential expression analysis, 950 and 1,452 differentially expressed genes were discovered under the conditions of 230-130mV and 120-N, respectively, and the sample 230-N was used as the control. These genes are mainly associated with the pathways of central carbon metabolism and ethanol formation. Increased expression of inulinase and the low activity of the autophagy-related gene, ATG8, ensured fast and ideal fermentation processes. Despite being reported as the “crabtree-negative” species, K. marxianus Y179 could achieve favorable ethanol fermentation profiles under micro-aeration and high inulin concentrations. K. marxianus Y179 cells responded to inulin concentrations and micro-aeration that is involved in the whole ethanol metabolism network. These results will serve as an important foundation for further exploration of the regulatory mechanisms involved in ethanol fermentation from inulin by consolidated bioprocessing and also provide a valuable reference for future studies on optimization and reconstruction of the metabolism network in K. marxianus. The online version of this article (doi:10.1186/s13068-015-0295-y) contains supplementary material, which is available to authorized users.